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Microarrays

Microarrays are utilized to study the expression of hundreds or even thousands of genes in a single experiment. A microarray is a solid support to which hundreds to thousands of individual cDNAs or DNA oligomers (each specific to a unique gene) have been immobilized. The cDNAs or oligomers are applied to the support in a grid pattern so that each individual cDNA has a unique position within the grid. mRNA is isolated from the experimental and control tissue or cells and cDNA is [Pg.16]


Bioinformatics Gene Expression Analysis Microarray Technology Pharmacogenetics Proteomics... [Pg.511]

DNA microarrays, or DNA chips consist of thousands of individual DNA sequences arrayed at a high density on a single matrix, usually glass slides or quartz wafers, but sometimes on nylon substrates. Probes with known identity are used to determine complementary binding, thus allowing the analysis of gene expression, DNA sequence variation or protein levels in a highly parallel format. [Pg.526]

There are many protocols and different types of platforms available, e.g. GeneChips from Affymetrix, Illumina Bead Arrays, Arrays from Agilent, Applied Biosystems, GE Healthcare, customized spotted cDNA microarrays etc. the basic procedure for a large-scale measurement of gene expression involves the preparation of total or mRNA from the biological sample(s) under investigation (e.g. candidate tissue) and the hybridization of copied labelled RNA or cDNA to the DNA elements on the array surface (Fig. 1). [Pg.526]

Microarray experiments generate large and complex data sets that constitute e.g. lists of spot intensities and intensity ratios. Basically, the data obtained from microarray experiments provide information on the relative expression of genes corresponding to the mRNA sample of interest. Computational and statistical tools are required to analyze the large amount of data to address biological questions. To this end, a variety of analytical platforms are available, either free on the Web or via purchase of a commercially available product. [Pg.527]

As a result of further technological improvements and decreasing costs, the use of microarrays will become an essential and potentially routine tool in understanding the mechanistic basis of action of many diugs. [Pg.528]

Target validation, to determine that a gene product is causative of disease symptoms or that activation of the target protein ameliorates disease symptoms. Agonist/activator or an inhibitor which may be therapeutic could be identified using microarrays... [Pg.528]

At present, the use of microarrays in pharmacology is especially established in the field of oncology by gene expression analysis with either tumour cell... [Pg.528]

MAQC Consortium (2006) The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements. Nat Biotechnol 24(9) 1151-1161... [Pg.529]

Microarray hybridization is a process by which nucleic acids are detected by hybridizing with complementary sequences bound to wafers at specific array coordinates. Hundreds to thousands of gene products may be measured in a single experiment. [Pg.765]

The most common application of microarray technology is in monitoring gene expression (gene expression profiling). [Pg.765]

A relatively large amount of RNA needs to be labeled with fluorescent dyes in order to obtain satisfactoiy results (20-100 pg of total RNA or 1-2 pg of mRNA). These requirements could make microarrays incompatible with the very limited RNA yields obtained from some... [Pg.767]


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Abundance-based microarrays

Affymetrix expression microarrays

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Affymetrix expression microarrays probes

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Amine microarray surfaces

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Microarray

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