Peptides microarray analysis

Lue, R. Y, Zhu, Q., Li, D. (2004). Site-specific peptide immobilization strategies for the rapid detection of kinase activity on microarrays. Site-specific immobilization of biotinylated proteins for protein microarray analysis. Enzymatic profiling system in a small-molecule microarray. Intein-mediated biotinylation of proteins and its application in a protein microarray. Developing site-specific immobilization strategies of peptides in a microarray. Methods Mol. Biol. 278, 191-204. 

Rychlewski L, Kschischo M, Dong L, Schutkowski M, Reimer U (2004) Target specificity analysis of the Abl kinase using peptide microarray data. J Mol Biol 336 307-311... 

Figure 7 Protein microarrays and their applications. Ligands, such as proteins, peptides, antibodies, antigens, allergens, and small molecules, are immobilized in high density on modified surfaces to form functional and analytical protein microarrays. These protein microarrays can also be used for various kinds of biochemical analysis.

In addition to commercially production, a great deal of research and development work on biochips has been going on both in industry and in academia. Genomic analysis of DNA and RNA continues to be the focus of interest, but more and more effort is being spent on proteomic analysis of proteins and peptides. Several enzyme assays and immunoassays designed based on microarray-based systems with simple microfluidic control are close to commercialization. They can be a vital tool in clinic diagnostics, drug discovery, and biomedical research. 

RG - reactive group, BG - binding group, tag - biotin and/or fluorophore. Probe-labeled proteomes can be analyzed via several different platforms, including gel [20] or microarray [26] analysis of probe-labeled enzymes, or capillary electrophoresis (CE) [28] and liquid chromatography-mass spectrometry (LC-MS) [27] analysis of probe-labeled tryptic peptides. 

New trends in the analysis of food contaminants in general focus on the simultaneous detection of different analytes present in the same sample. A multiplex PCR assay has been developed for the simultaneous detection of wheat, buckwheat, and peanut (Hashimoto et al., 2007). A distinct amplicon size for each allergen can be observed in an agarose gel. Since some RT-PCR thermocyclers have the ability to detect several fluorescent probes, they can easily be adapted to detect several food allergens simultaneously. Moreover, other platforms commonly used for multiplexing can be used in combination with PCR. Microarray slides can be spotted with peptide nucleic acid (PNA) capture probes for PCR products obtained from the amplification of DNA... 

Digital Gene Expression Profiling, edited by Kare Lehmann Nielsen, 2007 Peptide Characterization and Application Protocols, edited by Gregg B. Fields, 2007 Microchip-Based Assay Systems Methods and Applications, edited by Pierre N. Floriano, 2007 CapUlary Electrophoresis Methods and Protocols, edited by Philippe Schmitt-Kopplin, 2007 Cancer Genomics and Proteomics Methods and Protocols, edited by Paid B. Fisher, 2007 Microarrays, Second Edition Volume 2, Applications and Data Analysis, edited by Jang B. Rampal, 2007... 

Alternatively, microchip technologies offer other platforms for HTS such as microarrays and microfluidic devices. Microarrays allow the simultaneous analysis of thousands of parameters within a single experiment and for this reason have become cracial tools in drug discovery and life sciences research. They consist of immobilized biomolecules spatially addressed on planar surfaces, microchannels or microweUs, or an array of beads immobilized with different biomolecules. Biomolecules commonly immobilized on microarrays include oligonucleotides, polymerase chain reaction (PCR) products, proteins, lipids, peptides, and carbohydrates. Currently, in situ synthesized microarrays can be purchased or... 

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