Protein-binding microarray

Key Words Protein array biotinylation proteomics functional analysis surface capture p53 biotin carboxyl carrier protein fusion protein microarray DNA binding. 

In a protein-binding assay with fluorescence detection a microarray of biotin, HPYPP-peptide and WSHHPQFEK-peptide was screened against streptavidin-Cy3 and avidin-Cy5. By following the same principle an anti-human insulin monoclonal antibody was also screened against a set of different peptides. 

In the original report, it was used for the screening of protein binding. Like the OBOC method, a whole-cell binding assay also can be applied to the encoded planar chemical microarrays, although this has not been reported. 

Bulyk ML. Analysis of sequence specificities of DNA-binding proteins with protein binding microarrays. Methods Enzymol. 2006 410 279-299. 

Schmidt and co-workers have also immobilized the mannose derivatives to the commercially available glass slides under reductive amination or acylation conditions [167], Their study showed that the ConA interaction was in accordance with those obtained in aqueous solution and other immobilization methods, and non-specific protein binding to the functionalized glass slides was negligible. The same carbohydrate microarray plate could also be tested for other lectins, therefore the precious immobilized carbohydrates can be reused. 

Protein Microarrays Are Promising Tools for Monitoring Cell Responses That Include Changes in Protein-Binding Patterns... 

In a similar method, Lee and Shin (36) have developed hydrazide-coated glass slides to immobilize a wide range of carbohydrates including mono-, di-, and oligosaccharides in a simple, efficient, and chemoselective fashion. Preliminary protein-binding experiments show that carbohydrate microarrays prepared by this method are suitable for the high-throughput analysis of carbohydrate-protein interactions. 

In functional protein microarrays, set of proteins of interest or an entire proteome is over expressed, purified and spotted in an addressable microarray surface. The most obvious target molecules that can effectively be used to investigate protein binding and therefore protein microarray analysis are monoclonal antibodies or IgG. However, unlike DNA microarrays, antibody arrays may pose some practical concerns ... 

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