Microarray cell-binding

In our experience, the best support for cell-binding assays is a plastic slide. PVDF membrane has high adsorption capacity, and microarrays printed on such membrane are suitable for enzyme-linked colorimetric and phosphorylation assays. However, researchers are encouraged to try all of them for their specific applications. 

In the original report, it was used for the screening of protein binding. Like the OBOC method, a whole-cell binding assay also can be applied to the encoded planar chemical microarrays, although this has not been reported. 

The ultimate goal of microarray-based expression analysis is to acquire a comprehension of the entire cellular process, in order to exploit and to standardize the multidi-menisional relations between genotype and phenotype. However, an increasingly important parameter, which has not yet been substantially taken into account, is the role of cellular translation. This means that mRNA expression data need to be correlated with the assortment of proteins actually present in the cell. One approach is based on the use of microarrays containing double-stranded DNA probes for the analysis of DNA-protein interaction and, thus, the detection and identification of DNA-binding proteins by means of fluorescence [130] or mass spectrometry analysis [131]. Moreover, substantial efforts are currently under way to develop protein, antibody, or even cell arrays, applicable to the cor-... 

Egener, T., Roulet, E., Zehnder, M., Bucher, P. and Mermod, N. (2005) Proof of concept for microarray-based detection of DNA-binding oncogenes in cell extracts. Nucleic Acids Res. 33, e79. 

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