Experimental design microarray technology

Oxford Gene Technologies offers services and licenses their proprietary technologies. The array technique surveys hybridization across gene sequences. The customized DNA microarray service supports research activities, and includes consultation, experimental design, data analysis, and interpretation. 

This section describes the use of the microarray technology for transcriptional profiling. The section includes a summary of the experimental procedures (section on Experimental design), the most widely used array platforms (section on Platforms for Gene Expression Analysis), and briefly summarizes the data analysis steps, the software that can be used, and the public microarray data repositories (sections on Analysis of microarray data and Data sharing). 

In Sections 2 to 4, we review the technology of synthetic oligonucleotide microarrays and describe some of the popular statistical methods that are used to discover genes with differential expression in simple comparative experiments. A novel Bayesian procedure is introduced in Section 5 to analyze differential expression that addresses some of the limitations of current procedures. We proceed, in Section 6, by discussing the issue of sample size and describe two approaches to sample size determination in screening experiments with microarrays. The first approach is based on the concept of reproducibility, and the second approach uses a Bayesian decision-theoretic criterion to trade off information gain and experimental costs. We conclude, in Section 7, with a discussion of some of the open problems in the design and analysis of microarray experiments that need further research. 

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