Abundance-based microarrays

Abundance-based (analytical) microarrays, which seek to measure the abundance of specific biomolecules (e.g. proteins) using analyte-specific reagents such as monoclonal antibodies. For this purpose, capture microarrays are generated by spotting specific capture... 

Microarrays based on cDNA or oligonucleotides differ fundamentally in how their experimental outputs are analyzed. Typical cDNA arrays are hybridized with differentially labeled cDNA pools generated from two separate RNA sources. For example, RNA may be harvested from untreated cells grown under a standard set of conditions and the cDNA produced from this RNA pool may be labeled with one fluorescent dye. A second RNA sample is then harvested from the same cell type after it is treated with a chemical or grown under a different set of conditions, and the cDNA is labeled with a second dye. Once the two cDNAs can be distinguished by their labels, equal amounts are mixed and hybridized competitively to the same cDNA array. The ratio of one dye signal to the other at each probe will reflect relative differences in abundance between the two RNA samples for the gene represented. 

Gao WM, et al. Distinctive serum protein profiles involving abundant proteins in lung cancer patients based upon antibody microarray analysis. BMC Cancer 2005 5 110. 

Recent molecular studies have confirmed earlier culture-based work that describes the microbiota of the small intestine as comprising between 10 and 10 microorganisms per mL ileal fluid. Samples of ileal fluid collected from ileostomy patients analyzed using the HITCHIP gut microbiota 16S rRNA targeted microarray revealed a microbiota dominated by lactobacilli, streptococci, clostridia with a lower abundance of strict anaerobes including... 

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