Microarray results, display

Figure 16.10. Display of microarray results retrieved from FileMaker Pro. This example illustrates the results of a query for genes upregulated in a series of cancer cell lines with ratios coded by a red to green color map (Khan et al., 1998). (See color plate.)...

Hu et al. have prepared an antibody microarray with monoclonal antibodies against anti-AFP, anti-CEA, and RAH. Antibodies were dissolved in a glycerol and a SDS solution at a concentration of 0.10 mg/mL in a final volume of 0.20 pi for one spot in a diameter of 1.5 mm. The previously blocked arrays were incubated against the proteins—a mixture of a-fetoprotein (AFP), CEA, and human IgG. Then an aliquot of the labeled antibodies Sm3+ anti-AFP, Eu3+—anti-CEA, and Au-GAH was added at a concentration of 0.50 pg/ml. The results showed that each labeled antibody displayed clear signals and good reproducibility. Furthermore, they mixed two or more antibodies in one spot, which could also specifically and selectively detect each individual signal (40). 

Advances in microarray technology, with the use of cDNA or oligonucleotides immobilized in a predefined organization on a solid phase, have led to measurements of mRNA expression levels on a genome-wide scale (see chapter 3). The results of the measurements can be displayed on a plot (fig. 13.1) on which a row represents one gene at various times, a column the whole set of genes, and the time of gene expression... 

In this section, we illustrate various testing methods with multiple-testing procedures with two real data sets T-cell immune response data and platelet stody data. The former from microarray experiments is used for comparing two or multiple samples and the latter for paired samples. Programming examples for these data analyses can be found in the appendix. Parts of the results are displayed in Tables 4.4-4.6. 

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