Comparative genomic microarray analysis

The methods for genome-wide analysis of ribosomal association are conceptually different from those for analysis of a single mRNA (e.g., northern analysis). While in assays of a single gene by northern analysis it is simple to compare the distribution of an mRNA in different fractions, in a genome-wide assay, the mRNAs are usually compared relative to others within the fraction. Because of that, it is important to analyze by northern analysis several mRNAs that appeared to be affected to different extents, and to compare the relative effects among this group between the microarrays and the northern analysis. 

Pinkel, D., Seagraves, R., Sudar, D., Clark, S., Poole, I., Kowbel, D., et al. (1998) High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays. Nature Genet. 20,207-211. 

Smoot JC, Barbian KD, Van Gompel JJ, Smoot LM, Chaussee MS, Sylva GL, Sturdevant DE, Ricklefs SM, Porcella SF, Parkins LD, Beres SB, Campbell DS, Smith TM, Zhang Q, Kapur V, Daly JA, Veasy LG, Musser JM Genome sequence and comparative microarray analysis of serotype Ml 8 group A Streptococcus strains associated with acute rheumatic fever outbreaks. Proc Natl Acad Sci USA 2002 99 4668 1673. 

Microarray Comparative Genomic Hybridization for the Analysis of Bacterial Population Genetics and Evolution... 

Jolly, R. A. et al., Pooling Samples Within Microarray Studies A Comparative Analysis of Rat Liver Transcription Response to Prototypical Toxicants, Physiol. Genomics, 22, 346, 2005. 

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