Microarray hybridization

Microarray hybridization is a process by which nucleic acids are detected by hybridizing with complementary sequences bound to wafers at specific array coordinates. Hundreds to thousands of gene products may be measured in a single experiment. 

Microarray Hybridization Microarray Technology Microbial Resistance to Drugs Microfrlaments Microtubule... 

Spellman, P. T., Sherlock, G., Zhang, M. Q., Iyer, V. R., Anders, K., Eisen, M. B., Brown, P. O., Bot-stein, D. and Futcher, B. (1998), Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization , Mol Biol. Cell, 9, 3273-3297. 

The amount of mix to add depends on the experimental setting and the number of fractions collected. We typically add 70 pi of spike-in mix into an entire sucrose gradient, where each fraction receives the relative share from that amount. For example, 35 pi of the spike-in mix will be added to each fraction of a gradient that was divided into two, and 7 pi of this mix will be added to each fraction of a gradient that was divided into 10fractions. The added amounts should consider losses during purification steps and that a minimum of 0.2 ng of each spike is needed to yield sufficient signal in the microarray hybridization. 

Kiewe P et al (2009) Prediction of qualitative outcome of oligonucleotide microarray hybridization by measurement of RNA integrity using the 2100 Bioanalyzer capillary electrophoresis system. Ann Hematol 88 1177-1183. doi 10.1007/s00277-009-0751-5... 

Barker CS, Griffin C, Dolganov GM et al. Increased DNA microarray hybridization specificity using sscDNA targets. BMC Genomics 2005 6 57. 

Noerholm, M., Bruus, H., Jakobsen, M.H., Telleman, P., Ramsing, N.B., Polymer microfluidic chip for online monitoring of microarray hybridizations. Labchip 2004, 4, 28-37. 

Other extensions that have been made include the construction of a biochip in which a reverse transcription PCRprocess can be carried out [384] and the integration of PCR with capillary electrophoresis [385], DNA microarray hybridization [386] and sample preparation [387,388]. The speed of analysis, the ease of integrating different functions and the relative low costs of micro-PCR production are important advantages of the miniaturized PCR technique that suggest that this bioorganic microreactor device will be thoroughly implemented in many analytical labs. 

Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, Eisen MB, Brown PO, Botstein D, Futcher B. Comprehensive identification of cell cycle-regulated genes of the yeast Saccha-romyces cerevisiae by microarray hybridization. Mol. Biol. Cell 1998 9 3273-3297. 

Adey NB, Lei M, Howard MT et al (2002) Gains in sensitivity with a device that mixes microarray hybridization solution in a 25-microm-thick chamber. Anal Chem 74 6413-6417... 

Mcquain MK, Seale K, Peek J et al (2004) Chaotic mixer improves microarray hybridization. Anal Biochem 325 215-226... 

Before setting up a microarray hybridization, the labeled probes must be purified, concentrated, and resuspended in the hybridization buffer containing the appropriate salts, blocking agents, and detergents. 

Al-Khaldi, S.F., K.M. Myers, A. Rasooly, and V. Chizhikov. Genotyping of Clostridium perfringens toxins using multiple oligonucleotide microarray hybridization. Mol Cell Probes. 2004, 18(6), 359-367... 

Volokhov, D., A. Pomerantsev, V. Kivovich, A. Rasooly, and V. Chizhikov. Identification of Bacillus anthracis by multiprobe microarray hybridization. Diagn Microbiol Infect Dis. 2004, 49(3), 163-171... 

Figure 16.2. The process of microarray hybridization using printed DNA probes. A robotic printer deposits DNA in a regular array on a series of glass slides. After they are processed, the slides are hybridized to a mixture of two cDNA pools derived from test and reference samples that have been labeled with spectrally distinct fluorochromes. After stringency washes, the microarray is scanned in a laser-scanning device, and the image is processed to generate numerical data. (See color plate.)...

---