DNA microarrays method

Gerry NP, Witowski NE, Day J, Hammer RP, Barany G, Barany F. Universal DNA microarray method for multiplex detection of low abundance point mutations. J Mol Biol 1999 292 251-262. 

A range of molecular based techniques are available to detect major fungal crop pathogens, including PCR and DNA microarray methods. 

There are a number of techniques used for target identification. Radioligand binding was a common technique until recently. Now DNA microarrays, expressed sequence tags, and in silico methods are used. 

The combination of chromatin immunoprecipitation with DNA microarrays allows the genome-wide analysis of the distribution of an antigen. The immunoprecipitated DNA is quantitatively amplified, labeled and used to probe DNA microarrays. In principle ChIP-on-chip methods can be divided into two basic groups, depending on the content of the microarrays which are used (i) microarrays/promoter tiling arrays and (ii) genome tiling arrays. 

The characterisation of memory-related genes and proteins belongs to the hot spots of current memory research (D Agata and Cavallaro 2002). Respective studies in the field of aversive memories employ different molecular biological methods including in situ hybridisation (Ressler et al. 2002), differential display (Huang et al. 1998), subtractive hybridisation (Stork et al. 2001) and DNA microarrays (Kida et al. 2002). Most critical for the correct interpretation... 

One of the early concerns about the application of DNA microarray to toxicology has been how to properly compare experiments that use a wide variety of commercial and proprietary platforms, protocols, and analyses methods. The Health and Environmental Sciences Institute (HESI) of the International Life Sciences Institute (ILSI) has... 

Probe cDNA which is 500 5,000 bases long, is immobilized onto a solid surface such as glass using robot spotting. Then, it is exposed to a set of probes either separately or in a mixture. This method was developed at Stanford University and is called conventional DNA microarray. ... 

As the density of information derived from efforts to sequence, map and identify human genes increased, so did the demand for analytical tools capable of exploiting this information. DNA microarrays were developed in response to this demand. Southern(69) was the first to describe parallel, in situ ohgonucleotide synthesis as a means of generating oligonucleotide probe arrays on solid supports for highly parallel hybridization analysis. Southern s method uses standard nucleotide synthetic reactions to synthesize the oligonucleotides. The reactions are carried out in a movable chamber, which provides a physical barrier between the reaction chamber and the intended synthesis area. 

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