Glycoprotein microarrays

Adams EW, Ratner DM, Bokesch HR, McMahon JB, O Keefe BR, Seeberger PH. Oligosaccharide and glycoprotein microarrays... 

P.H. Oligosaccharide and glycoprotein microarrays as tools in HIV glycobiology glycan-de-pendent gpl20/protein interactions. Chem. Biol. 2004, 11, 739-740... 

In one of the first reports of a carbohydrate microarray, under-ivatized polysaccharides and glycoproteins were immobilized directly on nitrocellulose-coated glass slides (10). In a further demonstration of the feasibility of noncovalent immobilization of underivatized glycans, Willats et al. reported microarrays of glycoproteins, proteoglycans, and polysaccharides on oxidized black polystyrene slides (11). Modified neutral and aiuoiuc dex-tran polysaccharides have also been synthesized and printed on semicarbazide-coated glass slides (12). 

The myelin formation is most often studied using microscopic analysis (including electron microscopy) of the immunocytochem-istry for specific proteins, such as myelin basic protein (MBP), protein zero, myelin associated glycoprotein (MAG), and peripheral myelin protein 22 [107, 108]. The same markers can also be evaluated by other protein assays (e.g., enzyme-linked immunosorbent assay (ELISA) and western blot) or at mRNA level using for example PCR or microarray assays. 

---