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Microarray-based proteomics

Microarray-based proteomics, where antibodies or other molecules that have been arrayed onto a surface to seleetively eapture proteins, has been presented and covered in a number of reviews. [Pg.1353]

This book picks up the array technology journey from the mid-1990s with the introduction of microarray-based gene expression analysis. The global analysis of genes by microarrays has provided a fresh and exciting view of the cellular process. More importantly, it enabled others to consider similar utility in various "omic" fields. Hence, we have witnessed the emergence of protein arrays to address proteomics. [Pg.244]

The throughput of proteomics is currently much lower than that of RNA microarrays, largely due to the requirement of MS analysis, or similar technologies. However, the microarray-based ap-... [Pg.871]

Wingren, C. Steinhauer, C. Ingvarsson, J. Persson, E. Larsson, K. Borrebaeck, C. A., Microarrays based on affinity-tagged single-chain fv antibodies Sensitive detection of analyte in complex proteomes, Proteomics 2005, 5, 1281-1291... [Pg.245]

Ofir, K. Berdichevsky, Y. Benhar, I. Azriel-Rosenfeld, R. Lamed, R. Barak, Y. Bayer, E. A. Morag, E., Versatile protein microarray based on carbohydrate-binding modules, Proteomics 2005, 5, 1806-1814... [Pg.246]

In addition to commercially production, a great deal of research and development work on biochips has been going on both in industry and in academia. Genomic analysis of DNA and RNA continues to be the focus of interest, but more and more effort is being spent on proteomic analysis of proteins and peptides. Several enzyme assays and immunoassays designed based on microarray-based systems with simple microfluidic control are close to commercialization. They can be a vital tool in clinic diagnostics, drug discovery, and biomedical research. [Pg.162]

The focus of Chapters 11 and 12 is on measuring autoantibody expression for tissue-based proteomic research. Chapter 11 depicts the use of arrayed transcription factors to identify serum autoantibodies that may be used as biomarkers and reporters of aberrantly regulated transcription factors. Similarly, Chapter 12 presents a novel and useful method for detecting autoantibody expression to a wide variety of native intracellular antigens that are immobilized on a monoclonal antibody microarray. [Pg.232]

Miniaturized, Microarray-Based Assays for Chemicai Proteomic Studies of Protein Function... [Pg.133]


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See also in sourсe #XX -- [ Pg.1353 ]




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