Microarrays, gene profiling

Key Words Adrenergic function gene expression gene profiling microarray mRNA receptor transcription. 

Protein expression profiling (protein differential display) using microarrays is considered an important new tool for proteomic discovery. It is similar in concept and approach to the gene expression microarray for mRNA profiling. Sreekumar and Chinnaiyan (2002) describe a general approach for using the microarray to monitor protein expression in cancer and normal tissues. Here are the steps ... 

Seventy-six gene profile Prognostic for lymph node negative irrespective of HR status Oligonucleotide microarray Good prognosis versus Poor prognosis Fresh/frozen Rotterdam Assay-in development by Veridex, Warren, NJ)... 

We chose DNA microarrays, instead of proteomic studies, as the method to conduct global analyses of the stressed E. coli cells because this genomic technique allows for screening of all ca. 4,200 genes in E. coli simultaneously and could possibly identify peptides, which are typically too small to identify in most proteomic techniques. In addition, several other groups (51-54) had conducted DNA arrays at different conditions examining the effect of Zn(II) excess on the gene profile of E. coli, and the results from these other studies could serve as comparisons for our data. 

Cunningham M J, Liang S, Fnhrman S, et al. (2000). Gene expression microarray data analysis for toxicity profiling. Annals of the New York Acad. Sci. 919 52-67. 

Tu Y, Stolovitzky G, Klein U (2002) Quantitative noise analysis for gene expression microarray experiments. Proc Natl Acad Sci U S A 99 14031 14036 van de Vijver MJ, He YD, vant Veer LJ et al (2002) A gene-expression signature as a predictor of survival in breast cancer. N Engl J Med 347 1999 2009 van t Veer LJ, Dai H, van de Vijver MJ et al (2002) Gene expression profiling predicts clinical outcome of breast cancer. Nature 415 530-536... 

The most common application of microarray technology is in monitoring gene expression (gene expression profiling). 

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