RPMA reverse-phase protein microarrays

Key words Reverse phase protein microarrays, RPMA, Drug development, Drug discovery, Biomarkers,... 

The term reverse phase protein microarray was first coined in 2001 by Paweletz et al. (5) in the landmark paper which introduced the platform and demonstrated its utility for analyzing proteins derived from laser capture microdissected (LCM) prostate cancer. RPMAs have also been referred to by other names in the literature including lysate arrays, reverse phase lysate arrays, protein microarrays, and reverse phase protein arrays (6). A very nice historical perspective on the development of RPJMA technology is presented in a review by Mueller et al. (6). A detailed description of the methods for performing RPMA is beyond the scope of this chapter and would be redundant because there are... 

Figure 3.11 Reverse-phase microarray. In the streptavidin linked to reporter molecules such RPMA, proteins extracted from cellular lysates as QDs or enzymes such as HRP that carry are arrayed onto a nitrocellulose substrate out catalyzed reporter deposition for signal and probed with a primary antibody. In turn, a amplification. (Reprinted with permission biotinylated secondary antibody recognizes from Reference [51]. Copyright 2007 American the presence of the primary antibody. The Chemical Society.) biotinyl groups are then detected by...

---