Genomics DNA microarray

Key Words Pharmacogenomics biomarker genomics DNA microarray clinical trial design validation... 

Mukherjee et al. combined the analysis of TFs (Abfl, Rapl and Migl) binding to ds genomic DNA microarrays with ChIP-on-chip data, revealing a degree of overlap but also differences between the two experiments [14], It was clear that the data from protein-binding microarrays (PBMs)—as defined by Mukherjee et al.—provide valuable information, particularly for poorly understood TFs. 

Roberts, W. S., Davis, F., Higson, S. P. J. Scanning electrochemical microscopy of genomic DNA microarrays-study of adsorption and subsequent interactions. Analyst (Cambridge, U.K.) 2009, 134, 1302-1308. 

It should be possible to extend the DNA microarray-binding experiment to whole-genome analysis of transcription factor binding sites. The authors suggest that a microarray spotted with 12,000 one-kilobase sequences would span the entire Saccharomyces cerevisiae genome (Bulyk et al., 2001). Such an array could be used to characterize the sequence specificity of S. cerevisiae transcription factors. These experiments would be useful for predicting functions of previously uncharacterized transcription... 

Shalon, D., Smith, S. J., and Brown, P. O. (1996). A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome Res. 6, 639-645. 

Brown, P. O. and Botstein, D. (1999), Exploring the new world of the genome with DNA microarrays , Nature (Genetics Supplement), 21, 33-37. 

Chamber J et al. DNA microarrays of the complex human cytomegalovirus genome profiling kinetic class with drug sensitivity of viral gene expression. J Virol 1999 73 5757-5766... 

Genome-Wide Analysis of mRNA Polysomal Profiles with Spotted DNA Microarrays... 

Borucki, M.K., James Reynolds, D.R., Call, T.J., Ward, B.P., and Kadushin, J. (2005) Suspension microarray with dendrimer signal amplification allows direct and high-throughput subtyping of Listeria monocytogenes from Genomic DNA./. Clin. Microbiol. 43, 3255-3259. 

Demand for these tools is being driven by the need to identify genetic polymorphisms that may be associated with disease, as well as gene functions in human, animal, and microorganism genomes. The DNA microarray technology can be used to identify pathogens,... 

---