Lectins microarray

Pilobello KT, Krishnamoorthy L, Slawek D, Mahal LK. Development of a lectin microarray for the rapid analysis of protein... 

Pilobello KT, Slawek DE, Mahal LK. A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome. Proc. Natl. Acad. Sci. U.S.A. 2007 104 11534-11539. 

Hsu KL, Mahal LK. A lectin microarray approach for the rapid analysis of bacterial glycans. Nat. Rotoc. 2006 1 543-549. 

Microarray analysis provides a technology platform for massive, parallel analysis of protein-protein interactions (MacBeath and Schrieber, 2000 Joos et al 2002). The difficulty with protein microarray is that proteins do not behave as uniformly as nucleic acids. Protein function is dependent on a precise and fragile 3D structure that may be difficult to maintain in a microarray format A practical challenge posed by proteins derives from their relatively delicate tertiary stracture, which is susceptible to unfolding during microarray printing. In addition, the efficiency and specificity of protein-protein interactions are not nearly as standardized as nucleic acid hybridization. The use of lectin microarrays in glycoform analysis also encounters the similar problems. 

Meany, D. L., L. Hackler, H. Zhang, and D. Chan. 2011. Tyramide signal amplification for antibody-overlay lectin microarray A strategy to improve the sensitivity of targeted glycan profiling. /. Proteome Res. 10(3) 1425-31. 

Keywords Bio-affinity Biomarker Functional glycomics Lectin microarray... 

Lectin Microarray, an Advanced Technology for Glycan Profiling. 107... 

A previously-developed technique called serial lectin-affinity chromatography is available. Although the technique is almost identical to the lectin microarray in its essence, it lacks throughput and speed, because it utilizes open columns and... 

Basic Fabrication of the Lectin Microarray System 2.1 Equipment... 

LecChipT Ver.1.0 is a commercial product for the lectin microarray it contains 45 different lectins (for a list see [5] or [37]), which were carefully selected from a pool of 167 candidate lectins, taking into consideration their binding specificity, stability, and economy. Lectins are briefly classified based on monosaccharide... 

Many researchers using the lectin microarray take advantage of a confocal fluorescence detection principle, because the method has already been adapted for established systems of DNA microarray [18, 19]. The method is simple, and has therefore been widely used. However, it requires washing of the microarray surface after the binding reaction, because a dried sample is required for the confocal-type detection system. One useful application of the system is multicolor detection using, e.g., Cy3 and Cy5, as described later. 

Data analysis is a critical part of lectin microarray experiments, because the data obtained from each microarray analysis shows systematic variation in microarray quality, scanner detection stability, sample preparation reproducibility, and labeling efficiency. For this purpose, an initial step to normalize the obtained signal intensities is essential. In this section, the two fundamental procedures required for data analysis are described [42 4]. 

Table 1 Representative research papers reporting applications of the lectin microarray...

Survey of glycoantigens in cells Irom a-l,3-galactosyltransferase knockout pig using a lectin microarray (2010) Miyagawa et al. [53]... 

Lectin microarray profiling of metastatic breast cancers (2011) Fry et al. [60]... 

Glycome diagnosis of human induced pluripotent stem cells using the lectin microarray (2011) Tateno et al. [46]... 

Analyzing the dynamic bacterial glycome with a lectin microarray approach (2006) Hsu et al. [64]... 

---