Randomly ordered microarrays

Tips Self-Assembled, Randomly Ordered Microarrays... 

The first technical ditference between the DASL assay and traditional microarray assays is that a random priming technique is utilized, and as such an intact polyA tail is not required for efficient labeling. Other differences include a mechanism by which two oligonucleotide probes must recognize the RNA of interest in order to be extended, ligated, and later amplified via PCR. This technique leads to both high specificity and high sensitivity (due to the PCR reaction). 

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