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Microarrays binding patterns

Protein Microarrays Are Promising Tools for Monitoring Cell Responses That Include Changes in Protein-Binding Patterns... [Pg.615]

Whole influenza viruses have also been shown to bind to carbohydrate microarrays in a preliminary study (24). The observed binding pattern matched with the previous determined binding preferences. Influenza virus is one of the most dangerous infectious pathogens leading to hundred thousands of deaths per year worldwide... [Pg.398]

DNA on the same microarray. Any disparities between the two binding patterns will pinpoint sequences from the individual with possible abnormalities, enabling closet examination. [Pg.249]

Sapsford etal. (2001) examined microarray-based antibody-antigen binding kinetics in real time to determine the effect of spot size. Capture antibodies were immobilized in an array pattern onto silver-clad microscope slides. Antimouse IgG was directly attached to the surface or attached via neutravidin capture of the biotinylated antibody. Cy5-labeled mouse IgG capture was monitored based upon the signal generated from the excitation of an evanescent wave guide (slide) with a 635-nm laser source detection was achieved by a charge-coupled device (CCD) camera system. Both static and flow-through conditions were employed. [Pg.195]

Figure 10.2 This scientist is examining a microarray slide. The pattern of binding of the test samples, to the array of DNA on the slide, is displayed on the computer screen. Libraries of small chemicals can also be analyzed for their ability to bind to DNA or protein microarrays in a search for potential new drugs. Figure 10.2 This scientist is examining a microarray slide. The pattern of binding of the test samples, to the array of DNA on the slide, is displayed on the computer screen. Libraries of small chemicals can also be analyzed for their ability to bind to DNA or protein microarrays in a search for potential new drugs.
Direct peptide patterning PS-binding peptides with ROD, RGE, and biotin end-group BSA HUVEC Pin-microarrayer to print PS-binding peptides, BSA backfill 2009 [159]... [Pg.70]

Since microarrays are simply spots of DNA arrayed in a known pattern, they also can be used for DNA-DNA hybridization studies. This is the basis for using micro-arrays for comparative DNA hybridizations, single nucleotide polymorphism (SNP) discovery, and chromatin immunoprecipiation followed by microarray chip hybridization (ChIP on chip analyses) to identify transcription factor binding sites (discussed below). [Pg.32]

Microarrays [21] in the chip format, prepared by attachment of biopolymers to a surface in a spatially discrete pattern, have enabled a low-cost and high-throughput methodology for screening interactions involving these molecules. The most important advantage compared to classical methods is that microarrays allow for several thousand binding events to... [Pg.674]


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