Protein microarrays characterization

Y. Zhou, O. Andersson, P. Lindberg and Liedberg, B. (2004) Protein microarrays on carboxymethylated dextran hydrogels immobilization, characterization and application. Microchim. Acta., 147, 21-30. 

Organic dye materials represent the largest and best characterized class of probes used in all manner of fluorescent analysis. As an overall class, these dyes are used in almost all areas of biotechnology, including biosensing, cellular imaging, clinical immunofluorescence, and in DNA/protein microarrays (42-45). Several major structural classes of organic fluorophore span the UV-to-near-IR spectrum see Fig. 4. UV dyes are typically pyrene-based, naphthalene-based, and coumarin-based structures, whereas the Vis/near-IR dyes include a variety of... 

Ren L., Tsuru K., Hayakawa S., Osaka A. Synthesis and characterization of gelatin-siloxane hybrids derived through sol-gel procedure. J. Sol-Gel Sci Technol, 2001 21 115-121 Ren L., Tsuru K., Hayakawa S., Osaka A. Novel approach to fabricate porous gelatin-siloxane hybrids for bone tissue engineering. Biomaterials 2002 23 4765 773 Rietti-Shati M., Rouen, D., Mandelbaum, R.T. Atrazine degradation by Pseudomonas strain ADP entrapped in sol-gel glass. J. Sol-Gel Sci. Technol. 1996 7 77-79 Rupcich N., Goldstein A., Brennan J.D. Optimization of sol-gel formulations and surface treatments for the development of pin-printed protein microarrays. Chem. Mater. 2003 15 1803-1811... 

Our results demonstrated that the identified subsets of the activated protein kinases significantly increased the accuracy of clinical outcome predictions. Most notably in the study, we evaluated protein phosphorylation levels instead of total protein expression levels. Protein phosphorylation and dephosphorylation are well-characterized biochemical processes for protein kinases to conduct cellular signal transduction. Phosphorylation at certain tyrosine, serine, or threonine residues in kinases is a key step for their activation, and the measurement of these phosphorylations reflects their functional status in vivo. Thus, the protein kinase phosphorylation-based tissue microarray more accurately reveals the molecular mechanisms of breast cancers, and more accurately predicts the individualized survival and treatment response. 

Schweitzer B, Predki P, Snyder M. Microarrays to characterize protein interactions on a whole-proteome scale. Proteomics 2003 3 2190-2199. 

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