Screening of Chemical Microarrays

Biotinylated target protein followed by streptavidin-alkaline phosphatase are used to screen bead library positive beads turned dark upon adding of BCIP substrate. 

Protease is used to screen bead library for protease substrate positive beads in this fluorescent-quench assay fluoresce because the quencher is cleaved. 

Live cells are used to screen bead library for cell surface binding ligands positive beads are coated with a monolayer of cells. 

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