Microarray drug discovery

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Applying Genomic and Proteomic Microarray Technology in Drug Discovery... 

Role of gene expression microarrays in drug discovery... 

Figure 1.15 Role of microarrays in drug discovery process.

A number of commercial antibody-based microarrays for multiplexed cytokines analysis are now available (Beckman Coulter BD Biosciences Panom-ics Pierce S S Zyomyx and others). Cytokines are essentially biomarkers of cell injury, inflammation, and apoptosis. They are released by cells in culture in response to drug action (Turtinen et al., 2004) or are elevated in serum in various disease states. Moreover, numerous cytokines are involved in cellular response and many serve as dual effectors (Asao and Fu, 2000). As a result, anticytokine microarrays are being evaluated in drug discovery for off-target toxicity testing to replace standard ELISA plate formats. 

We may also find that Sabatini s reverse transfection method of creating "live cell" microarrays offers even greater advantage for drug discovery and development (Ziauddin and Sabatini, 2001). Their method, relying on arrayed cDNA expression vectors to transform adherent cells, provides localized real-time gene expression analysis of the putative gene product. 

Huels, C., Muellner, S., Meyer, H.E., and Cahill, D.J., The impact of protein biochips and microarrays on the drug development process. Drug Discovery Today, 7, S119-S124, 2002. 

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