Microarray spatially separable

OBOC combinatorial bead-libraries can be considered as chemical microarrays that are spatially separable but non-addressable. The identity of the chemical compound on the positive beads can be determined directly with an automatic sequencer if it is an N-terminally unprotected peptide, by mass spectroscopy, or through chemical encoding. i A synthetic scheme for the OBOC library is shown in Figure 2. Using the highly efficient split-mix synthesis method,literally hundreds of thousands to millions of compounds can be prepared within a week. The recent... 

Because each cell cluster expresses a particular protein, and cell clusters are spatially separated, the TCA can be considered a particular type of protein microarray. The TCA utilizes eukaryotic cells, allowing for PTMs of expressed proteins. Utilization of different cell lines provides opportunities to screen for protein function in situations where ceU-type-dependent PTMs and PPIs are important. Hence, the investigation of protein function within... 

For cDNA arrays, the effects of spatial artifacts and print tips are often accounted for using statistical models. Loess normalization can be performed separately for the genes in each technical block, microarray print tip (print-tip normalization), and sometimes even with different scaling factors (scaled print-tip normalization) (Yang et al., 2002). 

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