Carbohydrate microarray analysis

Manimala JC, Roach TA, Li ZT, Gildersleeve JC. High-thronghpnt carbohydrate microarray analysis of 24 lectins. Angew. Chem. Int. Ed. 2006 45 3607-3610. 

B) Chemoselective ligation for carbohydrate microarray analysis of protein-carbohydrate interactions D Rguiell... 

Karamanska R, Clarke J, Blixt O, MacRae JI, Zhang JQ, Crocker PR, Laurent N, Wright A, Flitsch SL, Russell DA, Field RA (2008) Surface plasmon resonance imaging for real-time, label-free analysis of protein interactions with carbohydrate microarrays. GlycoconJ J 25 69-74... 

Liu Y, Chai W, Childs RA, Feizi T. Preparation of neoglycol-ipids with ring-closed cores via chemoselective oxime-Ugation for microarray analysis of carbohydrate-protein interactions. Methods Enzymol. 2006 415 326-340. 

In a similar method, Lee and Shin (36) have developed hydrazide-coated glass slides to immobilize a wide range of carbohydrates including mono-, di-, and oligosaccharides in a simple, efficient, and chemoselective fashion. Preliminary protein-binding experiments show that carbohydrate microarrays prepared by this method are suitable for the high-throughput analysis of carbohydrate-protein interactions. 

M. Kawano, M.M. Thet, T. Makino, T. Kushida, and H. Sakagami, DNA microarray analysis of signaling pathway in macrophages stimulated by lignin-carbohydrate complex from Lentinus edodes mycelia (LEM) extract. Anticancer Res., 30 (7), 2567-2576,2010. 

Structural and conformational analysis which complements aspects in Section 4 of carbohydrate-protein interaction at the macromolecular level. This is due to an increased number of studies on the preparation of ligands for combinatorial chemistry and the design of carbohydrate microarrays (a recently popular activity coming of age along with genomic and proteomic methods). 

Alternatively, microchip technologies offer other platforms for HTS such as microarrays and microfluidic devices. Microarrays allow the simultaneous analysis of thousands of parameters within a single experiment and for this reason have become cracial tools in drug discovery and life sciences research. They consist of immobilized biomolecules spatially addressed on planar surfaces, microchannels or microweUs, or an array of beads immobilized with different biomolecules. Biomolecules commonly immobilized on microarrays include oligonucleotides, polymerase chain reaction (PCR) products, proteins, lipids, peptides, and carbohydrates. Currently, in situ synthesized microarrays can be purchased or... 

Barrangou, R., Azcarate-Peril, M.A., Duong, T., Conners, S.B., Kelly, R.M. and Klaenhammer, T.R. (2006) Global analysis of carbohydrate uiiMxaliotihy Lactobacillus acidophilus using cDNA microarrays. Proc Natl Acad Sci U SA 103, 3816-3821. 

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