The Use of DNA Microarray

Scientists have developed a DNA microarray test that predicts whether a woman with a certain type of breast cancer will have the cancer recur after the tumor is removed. This helps physicians and 

A large part of modern cancer research involves analyzing genes from diseased cells to determine which genes are turned on or turned off. By identifying a correlation between gene activity and a disease, - DNA strands — 

Each subdivision is 18 microns wide — about one-third the width of a strand of human hair. 

Single strands of RNA from a cancerous tissue sample are treated with a fluorescent formula and then dropped on the chip. Some of the RNA strands bind with complementary DNA strands on the chip, while others are washed away. Using a computer to observe areas of brightness on the chip s grid, scientists can determine which genes in the tumor are turned on. 

A glass plate about the size of a dime is divided into a grid of 

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In this review we focus on the use of DNA microarrays for the purpose of differential gene expression analysis. Discussions are intended to provide an overview of DNA microarray technology focusing on themes currently believed important to ensuring a successful DNA microarray experiment. Specifically, we address practical issues surrounding the use of DNA microarray technology with emphasis placed on its utility in the investigation of experimental stroke. 

The best examples for the use of DNA microarray in cancer profiling are the studies on breast cancer to uncover new disease subtypes. Based on the data... 

Simon R, Radmacher MD, Dobbin K, McShane LM. Pitfalls in the use of DNA microarray data for diagnostic and prognostic classification. J Natl Cancer Inst 2003 95 14-8. 

The growth in the use of DNA microarrays experienced in the past decade has been paralleled by the necessary developments in methodology, including new methods to model and analyze the data and new tools to implement these methods (16). 

The previous section looked at examples in which detailed analysis of specific proteins identified as targets of the heat shock response has led to the identification of new molecular chaperones as well as an expanded view of the requirements for efficient protein production and folding. In this section we focus on how the use of DNA microarrays has provided a global view of the spectrum of targets of a specific stress response, the unfolded protein response (UPR), which plays a critical... 

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