Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Gene expression microarrays labeled complementary sequences

Several methods are available to monitor transcription levels of tens of thousands of genes rapidly and simultaneously. The quantification of RNA species by sequence-specific annealing (hybridization) to complementary DNA probes arrayed on a substrate (microarray) was developed by Schena etal. (1995). Sample RNA was submitted to reverse transcription and fluorescent labeling. Thereby, a quantitative parameter was produced that could be measured as a localized signal after hybridization to the arrayed sensors. Comparison by competitive hybridization of two RNA samples labeled with two different dyes (Cy3 and Cy5) resulted in expression ratios of the two sources (e.g., of tumor and nontumor tissue). Whereas sensors were originally taken from libraries of DNA clones (cDNA), present-day microar-... [Pg.301]


See other pages where Gene expression microarrays labeled complementary sequences is mentioned: [Pg.399]    [Pg.432]    [Pg.80]    [Pg.169]    [Pg.530]    [Pg.327]    [Pg.466]    [Pg.397]    [Pg.2796]    [Pg.366]    [Pg.327]    [Pg.397]    [Pg.208]    [Pg.667]    [Pg.67]    [Pg.157]    [Pg.249]    [Pg.146]    [Pg.1746]    [Pg.1781]    [Pg.85]    [Pg.32]    [Pg.17]    [Pg.630]    [Pg.152]    [Pg.7]    [Pg.44]    [Pg.61]   
See also in sourсe #XX -- [ Pg.14 ]




SEARCH



Complementariness

Complementary

Complementary sequence

Gene expression microarrays microarray

Gene microarrays

Gene sequences

Microarray

Microarrays

© 2024 chempedia.info