Microarrays frozen protein

Schoenberg Fejzo, M. and D. J. Slamon. 2001. Frozen tumor tissue microarray technology for analysis of tumor RNA, DNA, and proteins. Am J Pathol 159 1645-50. 

Methods are under development for analysis of protein from formalin-fixed tissues, although protein yield may be less than that from frozen tissue. Extraction of high-yield, quality protein from formalin-fixed tissue is generally difficult because of the extensive cross-links formed in formalin-fixed samples. Currently, the optimal tissue specimen for use with protein lysate microarrays is fresh tissue, immediately embedded in a cyroprotectant solution and frozen or snap-frozen tissue. 

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