DNA microarray technology

The cDNA technology is essentially an array-based, high-throughput protocol that determines gene expression and copy number survey of very large numbers of tumors. As many as 1,000 cylindrical tissue biopsies from individual tumors can be distributed in a single tissue microarray. Sections of the microarray also provide targets for parallel in situ 

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Khan J et al DNA microarray technology the anticipated impact on the study of human disease. Biochim Biophys Acta 1999 1423 M17. 

Lettieri, T., Recent applications of DNA microarray technology to toxicology and ecotoxicology,... 

Al-Khaldi, S. F. Martin, S. A. Rasooly, A. Evans, J. D. DNA microarray technology used for studying foodborne pathogens and microbial habitats Minireview. J. AOAC Int. 2002,85, 906-910. 

Afshari CA et al. Application of complementary DNA microarray technology to carcinogen identification, toxicology, and drug safety evaluation. Cancer Res 1999 59 4759-4760. 

Demand for these tools is being driven by the need to identify genetic polymorphisms that may be associated with disease, as well as gene functions in human, animal, and microorganism genomes. The DNA microarray technology can be used to identify pathogens,... 

Levels of RNA (usually specific mRNAs) in a cell can be measured by well-established techniques such as northern blot analysis or by PCR analysis. However, the recent advent of DNA microarray technology has converted the identification and measurement of specific mRNAs (or... 

In this review we focus on the use of DNA microarrays for the purpose of differential gene expression analysis. Discussions are intended to provide an overview of DNA microarray technology focusing on themes currently believed important to ensuring a successful DNA microarray experiment. Specifically, we address practical issues surrounding the use of DNA microarray technology with emphasis placed on its utility in the investigation of experimental stroke. 

Pathogenesis following stroke most likely reflects the integrative actions of multiple functional pathway acting in concert. Today, DNA microarray technology provides the opportunity for even the small-scale research laboratory to study complex interactions, like those occurring following cerebral ischemia. By... 

Cleary, J.D., Rogers, P.D., and Chapman, S.W., Differential transcription factor expression in human mononuclear cells in response to amphotericin B identification with complementary DNA microarray technology. Pharmacotherapy, 21, 1046-1054, 2001. 

Seizinger, B., and Loferer, H., DNA microarray technology and antimicrobial drug discovery, Pharmacogenomics, 1, 169-178, 2000. 

Koizumi, Y. Kelly, J.J. Nakagawa, T. Urakawa, H. El-Fantroussi, S. Al-Muzaini, S. Fukui, M. Urushigawa, Y. Stahl, D.A. Parallel characterization of anaerobic toluene- and ethylhenzene-degrading microhial consortia hy PCR-denaturing gradient gel electrophoresis, RNA-DNA membrane hybridization, and DNA microarray technology. Appl. Environ. Microbiol. 2002, 68, 3215-3225. 

Two different forms of the DNA microarray technology exist at present. 

Applications of DNA and Protein Microarray Technology DNA microarray technology can be applied for the following purposes. 

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