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Gene microarrays hybridization

Microarray hybridization is a process by which nucleic acids are detected by hybridizing with complementary sequences bound to wafers at specific array coordinates. Hundreds to thousands of gene products may be measured in a single experiment. [Pg.765]

Spellman, P. T., Sherlock, G., Zhang, M. Q., Iyer, V. R., Anders, K., Eisen, M. B., Brown, P. O., Bot-stein, D. and Futcher, B. (1998), Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization , Mol Biol. Cell, 9, 3273-3297. [Pg.347]

Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, Eisen MB, Brown PO, Botstein D, Futcher B. Comprehensive identification of cell cycle-regulated genes of the yeast Saccha-romyces cerevisiae by microarray hybridization. Mol. Biol. Cell 1998 9 3273-3297. [Pg.1852]

BioMicro Systems—Microarray hybridization, Phalanx Biotech—Human and mouse gene expression microarrays etc. [Pg.373]

Our data indicate that the formation of somatic globular embryos is accompanied by the accumulation of storage protein transcripts. Figure 9.6 illustrates that transcripts for the synthesis of gibberellic acid also accompany the formation of somatic embryos. These microarray hybridizations experiments have been entered into the GEO (Gene Expression Omnibus) database of NCBI at http //www.ncbi.nlm.nih.gov/geo. For complete details on interpretation of the data, see Thibaud-Nissen et al ... [Pg.189]

Sp>eUman, P. T., G. Sherlock, et al. (1998). "Comprehensive identification of ceU cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization." Mol Biol Cell 9(12) 3273-3297. [Pg.241]

Wilson, M. DeRisi, J. Kristensen, H.H. Imboden, R Rane, S. Brown, P.O. Schoolnik, G.K. Exploring Drag-Induced Alterations in Gene Expression in Mycobacterium tuberculosis by Microarray Hybridization. PNAS USA. 1999, 96, 12833-12838. [Pg.209]

There are many protocols and different types of platforms available, e.g. GeneChips from Affymetrix, Illumina Bead Arrays, Arrays from Agilent, Applied Biosystems, GE Healthcare, customized spotted cDNA microarrays etc. the basic procedure for a large-scale measurement of gene expression involves the preparation of total or mRNA from the biological sample(s) under investigation (e.g. candidate tissue) and the hybridization of copied labelled RNA or cDNA to the DNA elements on the array surface (Fig. 1). [Pg.526]

Kasamatsu A, Endo Y, Uzawa K, Nakashima D, Koike H, Hashitani S, et al. Identification of candidate genes associated with salivary adenoid cystic carcinomas using combined comparative genomic hybridization and oligonucleotide microarray analyses. Int J Biochem Cell Biol 2005 37 1869-80. [Pg.163]


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