Microarray gene expression experiments control

Microarray experiment An experiment to study a microarray system under controlled conditions while some conditions are changed. In gene expression, one parameter is varied, such as time, drug, developmental stage, or dosage on a sample. The sample is processed and labeled with a detectable tag so that it can be used in hybridization with microarray. 

In a recent publication, the largest number of AD brains to date were analyzed by microarray. Unlike the previous studies, the RNA from 61 AD frontal cortex samples and 53 controls samples were pooled, and multiple hybridization experiments took place on two-array platforms. Only 3 of the top 30 differentially expressed genes from each platform were found to be in common RGS4 and RAB3A were decreased and ITPKB was increased. However, real-time PCR with the individual samples confirmed these changes were specific to the AD patients, and these data support the evidence of disrupted intracellular calcium signaling in AD [112],... 

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