Microarray protein

Hosokawa, Y, Matsumura, S., Masuhara, H., Ikeda, K., Shimo-oka, A. and Mori, H. (2004) Laser trapping and patterning of protein microcrystals Toward highly integrated protein microarrays. J. Appl. Phys., 96, 2945-2948. 

Figure 7.7. Protein microarray on polyvinylidene difluoride filters (PVDF). Proteins are gridded on filter and probed with an antibody to a gene of interest. Protein-ligand interactions could be detected using a labeled ligand as a probe.

The potential of photolithography for the construction of protein microarrays has also been demonstrated (Mooney et al., 1996). For these experiments, antibodies were assembled in precise two-dimensional patterns on silicon wafers. This was accomplished by first forming a self-assembled monolayer of n-octadecyltrimethoxysilane (OTMS) on a silicon-dioxide... 

Lueking, A., Horn, M., Eickhoff, H., Bussow, K., Lehrach, H., and Walter, G. (1999). Protein microarrays for gene expression and antibody screening. Anal. Biochem. 270, 103-111. 

Ikeda, K., Nakazawa, H., Shimo-Oka, A. et al. (2006) Immobilization of diverse foreign proteins in viral polyhedra and potential application for protein microarrays. Proteomics, 6 (1), 54—66. 

Kodadek, T. (2001), Protein microarrays prospects and problems , Chem. Biol., 8, 105 -115. 

Armando Aranda I think the next stage is actually the protein microarrays. Scientists got behind the times and they thought of it too late, but they are busy developing them and I think within eighteen months there will be several protein microarrays on the market. 

Haab BB Advances in protein microarray technology for protein expression and interaction profiling. Curr Opin Drug Discovery Dev 2001 4 116-123. 

Ramachandran N, Hainsworth E, Bhullar B, et al. Self-assembling protein microarrays. Science 2004 305 86-90. 

Weller M.G., Classification of protein microarrays and related techniques, Anal Bioanal Chem. 2003 375 15-17. 

Peluso P., Wilson D.S., Do D., Tran H., Venkatasubbaiah M., Quincy D., Heidecker B., Poindexter K., Tolani N., Phelan M., Witte K., Jung L.S., Wagner P., Nock S., Optimizing antibody immobilization strategies for the construction of protein microarrays, Anal Biochem. 2003 312 113-124. 

Angenendt P., Gloekler J., Sobek J., Lehrach H., Cahill D.J., Next generation of protein microarray support materials evaluation for protein and microarray appbcations, J. Chromatography A 2003 1009 97-104. 

Schaeferling M., Schiller S., Paul H., Kruschina M., Pavlickova P., Meerkamp M., Giammasi C., Kambhampati D., Application of self-assembly techniques in the design of biocompatible protein microarray surfaces, Electrophoresis 2002 23 3097-3105. 

Currently, protein microarrays can be divided into various types depending on the strategies to be chosen. For example, according to the array structure and shape, protein microarrays include 3D-surface structure [30, 31], nanowell [32], and plain chips [33-36], Meanwhile, considering the field of application, protein microarrays can be classified into five categories antibody array, antigen or reserve array, functional array, capture array, and solute array. Table 11.6 shows the differences among them. 

The most common use of protein microarrays is in immunoassays. In particular, antibody-based immunoassays are the main stream of diagnostic assays due to their specificity. The assay usually runs in a multiplexed mode where the antibodies or other capture agents are immobilized and then exposed to a biological sample. There are four immunoassay formats direct binding, sandwich (ELISA), competitive, and displacement. Direct-binding and sandwich assays are the most common. There are some reports on the use of competitive assays and displacement assays, which are usually associated with high surface area/volume systems [72-76],... 

L.O. Lomas, Protein Microarray Technology, Chap. 8, Wiley-VCH (2004). 

A. Sreekumar, M.K. Nyati, S. Varambally, T.R. Barrette, D. Ghosh, T.S. Lawrence, and A.M. Chinnaiyan, Profiling of cancer cells using protein microarrays discovery of novel radiation-regulated proteins. Cancer Res. 61, 7585-7593 (2001). 

C.P. Paweletz, L. Charboneau, V.E. Bichsel, N.L. Simone, T. Chen, J.W. Gillespie, M.R. Emmert-Buck, M.J. Roth, E.F. Petricoin, and L.A. Liotta, Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front. Oncogene 20, 1981-1989 (2001). 

A.J. Sinskey, S.N. Finkelstein, and S.M. Cooper, The promise of protein microarrays. PharmaGenomics... 

Benters, R. Niemeyer, C. M. Wohrle, D., Dendrimer activated solid supports for nucleic acid and protein microarrays, Chembiochem. 2001, 2, 686 694... 

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