Microarray analysis targets

Chang YE et al. Microarray analysis identifies interferon-inducible genes and Stat-1 as major transcriptional targets of human papillomavims type 31. J Virol 2000 74 4174-4182. 

Microarray analysis of Potential Transcription Factor Targets... 

Oligonucleotide microarray analysis of bone marrow samples from patients with IM-sensitive and IM-resistant CML have shown the ability to distinguish IM sensitivity and resistance. Differential gene expression patterns have the potential to identify new gene and protein targets for treatment and may have the potential to be used as a screening tool to identify patients with resistance prior to therapy (68,69,70,71,72). 

The dysregulation of transcription has provided a target for therapeutic intervention, and microarray analysis has been used to follow the effect of the drug in the treated samples. For example, acetylation of histones regulates... 

Lock C, Hermans G, Pedotti R, Brendolan A, Schadt E, Garren H, et al. Gene-microarray analysis of multiple sclerosis lesions yields new targets validated in autoimmune encephalomyelitis. Nat Med 2002 8(5) 500-508. 

Weinmann AS, Yan PS, Oberley MJ, Huang TH, Farnham PJ. Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis. Genes Dev 2002 16(2) 235-244. 

Hertzberg M, Sievertzon M, Aspeborg H, Nilsson P, Sandberg G, Lundeberg J. cDNA microarray analysis of small plant tissue samples using a cDNA tag target amplification protocol. Plant J. 2001 25 585-591. 

Cristillo AD, Bierer BE. Identification of novel targets of immunosuppressive agents by cDNA-based microarray analysis. J Biol Chem 2002 277 4465-76. 

Cherkaoui-Malki, M., K. Meyer, W.-Q. Cao, N. Latruffe, A.V. Yeldandi, M.S. Rao, C.A. Bradfield and J.K. Reddy. Identification of novel peroxisome proliferator-activated receptor a (PPARa) target genes in mouse liver using cDNA microarray analysis. Gene Express. 9 291-304, 2001. 

A. Microarray Analysis of Sulfur Mustard Exposure Reveals Potential Therapeutic Targets Coneirmed by Protein Analysis... 

Fig. 1. A standard approach to microarray analysis using spotted cDNA arrays. Target DNA may alternatively consist of synthetic oliogonucleotides. In some applications a single probe population is hybridized per slide.

Fig. 2. Resultant scan from a cDNA microarray analysis of Stat3-specific gene targets in mammary epithelial cells. (A) Portion of a 15,000 mouse cDNA array, hybridized with Cy3 (inactive Stat3) and Cy5 (active Stat3)-labeled cDNA probes from a mouse mammary epithelial cell line. Note the range of hybridization intensities between cDNAs spots on the array, representing relative differences in the expression of these transcripts in the complex probe populations. (B) and (C) Differential hybridization to individual cDNAs on the same part of the array is evident in separate scans of Cy-3 (B) and Cy-5 (C) labeled probes. These cDNA clones therefore represent likely gene targets of the Stat3 transcription factor.

---