PE Applied Biosystems

The PCR primers and the fluorogenic probes for the studied targets were designed using the Primer Express Software 1.7 (PE Applied Biosystem, Foster City, CA, USA). To normalize the quantitative data, specific probes for the TATA-bind-ing protein mRNA were used as an internal control. 

PE Applied Biosystems ABI 433A Boc/Fmoc vortex 0.1 -l.Ommol feedback monitoring... 

Novex (San Diego, G ). ProSorb Cartridges, ProBlott PVDF membrane and all the solvents and reagents used for HPLC were obtained from PE Applied Biosystems (Foster City, CA). 

Fig. 2. A schematic diagram of PE Applied Biosystem s 173A capillary HPLC/MicroBlotter System illustrating the woiidng principles for peptides separation and collection.

Sequencing of the generated CNBr fragments was performed on PE Applied Biosystems Procise cLC Sequencer. 50 mg of polybrene in a 70% methanolic solution was loaded onto each excised membrane prior to sequencing. Sequencing was accomplished using the Gas-Phase cLC method. 

Perkin-Elmer Corp. / PE SCIEX / PE Applied Biosystems 761 Main Ave., Norwalk, CT 06859, USA Phone (203) 762-1000 Fax (203) 762-6000 Internet http //www.perkin-elmer.com http //www. pesciex.com Stock PKN (NYSE)... 

PE Applied Biosystems R. W. Johnson Pharmaceutical Research Institute Karo Bio Inc. 

PE Applied Biosystems www2.perkin-elmer. com/ab PerSeptive Biosystems... 

PE Applied Biosystems www2.perkin-elmer. com/ab 3948 nucleic acid synthesis and purification system DNA 48 10-60 nmol Gas pressure No On-line cleavage, purification, and optical density measurement... 

PE Applied Biosystems SOLARIS 530 organic 48 7 Yes Off-line incubation ... 

Dr. Cheryl Heiner at PE- Applied Biosystems Advanced Center for Genetic Technology has developed a ThermoFidelase-based protocol for direct sequencing from genomic DNA. In the Heiner protocol, the following reaction setup is used. 

PE Applied Biosystems Peptide Synthesis Technical Bulletin. Feedback monitoring kit for ABI431A peptide synthesizer. 

PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA Kelvin Close, Birchwood Science Park North, Warrington, Cheshire, WA3 7PB, UK. 

GeneAmp PCR 0.5 ml reaction tubes (PE Applied Biosystems, N801-0180)... 

GeneAmp dNTPs separate 10 mM stock solutions of dATP, dCTP, dGTP, and dlTP in distilled water, titrated to pH 7.0 with NaOH (PE Applied Biosystems, N808-0007)... 

Figure 4. The emission spectra of four commonly used fluorescent dyes in automated DNA analyzers. Image courtesy of PE Applied Biosystems...

Figure 2 Mass spectra of VKl. (A) Parent ion spectrum, generated by APCI interface and quadrupole MS technology. (B) Daughter ion spectrum after fragmentation (25 eV) of parent ion m/z = 451 (MH ) using quadrupole MSMS technology. (The spectra are recorded in the German PE Applied Biosystems application labs on an APB65LCMSMS instrument.)...

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