Oligosaccharides microarrays

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Zhou X, Zhou J. Oligosaccharide microarrays fabricated on aminooxyacetyl functionalized glass surface for characterization of carbohydrate-protein interaction. Biosens. Bioelectron. 2006 21 1451-1458. 

Bran MA, Disney MD, Seeberger PH. Miniaturization of micro-wave-assisted carbohydrate functionalization to create oligosaccharide microarrays. ChemBioChem 2006 7 421-424. 

Many of the aforementioned carbohydrate microarray strategies have been used for observations on proteins of known specificities and their interactions with sugars. We highlight below several oligosaccharide microarray systems that are beyond proof-of-concept and have provided new biological insights. We dwell, in some detail, on the NGL-based oligosaccharide microarray platform with which we have first hand experience. 

Figure 5 Fluorescence image of oligosaccharide microarrays spotted with 10 ohgosaccha-ride probes with identical carbohydrate chips that were separately incubated with each of 3 biotin-labeled lectins (0.1 mg/ml in PBST) for 1 h, washed with PBST 3 times for 5 min each, and stained with 5 pg/ml of Cy3-strptavidin and evaluated by confocal fluorescence microscopy. Fluorescence images of oligosaccharide microarrays probed with (A) concanavah A (Con A), (B) LoTus Tetragonolobus (LT), and (C) Erythrina Gristagalli (EC).

Figure 7 Quantitative inhibition assays in oligosaccharide microarray. (A) Determination of the concentration of soluble a-methyl mannose to inhibit 50% of Con A binding to spotted mannose. (B) Determination of the concentration of a-methyl mannose to inhibit 50% of Con A binding to spotted glucose. Each data point represents the meaniSD for 10 spots from 2 independent experiments.

Hirabayashi J (2003) Oligosaccharide microarrays for glycomics. Trends Biotechnol 21 141-143 discussion 143... 

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