Microarray preparation

Lee Y, Lee EK, Cho YW, et al. (2003) Pro-teoChip a highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein-protein interaction studies. Proteomics 3, 2289-304. 

Microarrays prepared from macromolecular scaffold conjugates were found to be stable in all washing procedures used in our laboratory. However, mechanical wiping must be avoided, as it will damage the microarrays. 

Bordoni, R., Consolandi, C., Castiglioni, B., Busti, E., Bemardi, L. R., Battaglia, C., and Beilis, G. D. (2002). Investigation of the multiple anchors approach in oligonucleotide microarray preparation using linear and stem-loop structured probes. Nucleic Acids Research 30 34. 

TABLE 1 Comparison of Three Bifunctional Linkers Used for Microarray Preparation ... 

On-slide quantification Application of conjugated glycans Yes Coupled to activated glass slides, proteins, and microspheres (38) No Used for various glycan microarray preparation (40,42-44) No Used to prepare microarrays of neoglycoproteins and glycosphingolipids (41,45)... 

Reed MR, Coty WA. eSensor a microarray technology based on electrochemical detection of nucleic acids and its application to cystic fibrosis carrier screening, in microarrays preparation, detection methods, data analysis, and applications. In Dill K, Liu R, Grodzinski P, editors. Kluwer Springer-Verlag 2008 in press. 

In a similar method, Lee and Shin (36) have developed hydrazide-coated glass slides to immobilize a wide range of carbohydrates including mono-, di-, and oligosaccharides in a simple, efficient, and chemoselective fashion. Preliminary protein-binding experiments show that carbohydrate microarrays prepared by this method are suitable for the high-throughput analysis of carbohydrate-protein interactions. 

Figure 11 Carbohydrate microarrays prepared by covalent immobilization of maleimide-derivatized carbohydrates onto a thiol-functionalized substrate surface.

FIGURE 5.3.5 (a) Biotinylation of HL-60 cells and cell microarray preparation, (b) Cell microarray formed on an avidin-patterned surface. The cells were stained with calcein AM. Scale bar represents 100 xm. 

Robbins, M. E. Oh, B. K. Hopper, E. D. Schoenfisch, M. H., Nitric Oxide-Releasing Xerogel Microarrays Prepared with Surface-Tailored Poly(dimethylsiloxane) Templates. Chem. Mater. 2005,17,3288-3296. 

Estimation of results is done by measuring the intensity of fluorescence, which corresponds to the amount of gene expressed in the sample (Pandey et al. 2003 Labana et al. 2005). The three major steps of a microarray technology are preparation of microarray, preparation of labeled probes, and hybridization and finally, scanning, imaging, and data analysis (Labana et al. 2005 Esteve-Nunez et al. 2001). 

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