Microarray concordance

Figure 6.17 Concordance of standard ELISA and microarray ELISA formats in PSA determinations. (From Wiese, R. et al., Clin. Chem., 47,1451-1457, 2001. With permission.)...

Microarray analyses of breast cancer have identified unique gene expression profiles associated with patient survival. Sorlie et al. (23) found the expression profiles to distinguish ER-i- from ER- tumors with distinct outcomes in a cohort with locally advanced breast cancer treated with primary chemotherapy. Van t Veer et al. (18) established a 70-gene signature to predict metastatic potential in an untreated, node-negative cohort. Sotiriou et al. (24) showed the concordance with these previous analyses in node-positive and node-negative patients with the majority receiving adjuvant treatment. 

Another unique approach to microarray technology uses fiber optic bundles to support an array of microbeads that are coated with DNA capture sequences. Each bead is individually associated with a single fiber in the bundle, and hybridization targets labeled with fluorescent dyes are hybridized to the beads. The one-to-one concordance of bead to fiber in the bundle permits every bead in the hybridization array to be addressed and reported individually/94,95)... 

Our microarray data set contains substantial information about the mechanism and extent of CS effects on various genes. The small number of distinct temporal patterns indicates that a limited number of mechanisms may mediate CS pharma-cogenomics. Of special note, several genes such as ornithine decarboxylase and hydroxysteroid sulfotransferase were represented by multiple different probes on the gene arrays. Reasonable concordance in prohles and dynamic parameters from multiple probes indicated a good degree of reproducibility in results. 

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