Microarrays carbohydrate

Carbohydrates are key components of cell-surface glycolipids and glycoproteins, which play an important role in cellular functions such as cell adhesion, fertilization, 

4 One-Bead-One-Compound Combinatorial Library Bead-Arrays 

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T. Feizi, F. Fazio, W. Chai, and C.-H. Wong, Carbohydrate microarrays—anew set of technologies at the frontiers of glycomics, Curr. Opin. Struct. Biol., 13... 

M. D. Disney and P. H. Seeberger, The use of carbohydrate microarrays to study carbohydrate-cell interactions and to detect pathogens, Chem. Biol., 11 (2004) 1701-1707. 

Wang, D., Liu, S., Trummer, B.J., Deng, C., and Wang, A., Carbohydrate microarrays for the recognition of cross-reactive molecular markers of microbes and host cells, Nat. Biotechnol20, 275-281, 2002. 

The role that carbohydrates exert in recognition phenomena of biological and pharmaceutical relevance has stimulated the quest for tools that can monitor and even quantitatively evaluate these interactions. The potential diagnostic applications of such tools is evident, and the possible development of commercial carbohydrate microarrays, such as those established for oligonucleotides, and very recently peptides, is of high interest. 

The printed carbohydrate microarrays are air-dried and stored at room temperature without desiccant before further use (see Note 5). 

The staining procedure for carbohydrate microarrays is basically identical to the routine procedure for immunohistology. Immunostaining steps of carbohydrate arrays are listed below (see Notes 4 and 6 and Fig. 3). 

Ruobing Wang Carbohydrate Microarray Laboratory, Departments of Genetics, Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA... 

Zhi ZL, Powell AK, Turnbull JE (2006) Fabrication of carbohydrate microarrays on gold surfaces direct attachment of noderivatized oligosaccharides to hydrazide-modified self-assembled monolayers. Anal Chem 78 4786-4793... 

Karamanska R, Clarke J, Blixt O, MacRae JI, Zhang JQ, Crocker PR, Laurent N, Wright A, Flitsch SL, Russell DA, Field RA (2008) Surface plasmon resonance imaging for real-time, label-free analysis of protein interactions with carbohydrate microarrays. GlycoconJ J 25 69-74... 

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