Glycan microarrays

Analytical tools have been developed in order to identify carbohydrate structures as well as carbohydrate-binding proteins and to understand their underlying structure-function relationships of protein-carbohydrate and carbohydrate-carbohydrate interactions lectin arrays [16], glycan microarrays [17, 18], glyco-nanoparticles [19], frontal affinity chromatography [20] and carbohydrate tools for metabolic labeling [21]. 

Recent detection methods for glycan array include fluorescent assay, SPR, MALDI-TOF mass spectrometry, and nanoparticle assay. Fluorescence-based measurement is the prevalent principle for detecting binding to glycan microarrays. Rhodamine [9],... 

Dyukova VI, Dementieva El, Zubtsov DA, Galanina OE, Bovin NV, Rubina AY. Hydrogel glycan microarrays. Anal. Biochem. 2005 347 94-105. 

Nimrichter L, Gargir A, Gortler M, Altstock RT, Shtevi A, Weisshaus O, Fire E, Dotan N, Schnaar RL. Intact cell adhesion to glycan microarrays. Glycobiology 2004 14 197-203. 

On-slide quantification Application of conjugated glycans Yes Coupled to activated glass slides, proteins, and microspheres (38) No Used for various glycan microarray preparation (40,42-44) No Used to prepare microarrays of neoglycoproteins and glycosphingolipids (41,45)... 

Wang et al. reported the first glycan microarray in early 2002 (56). Since then, there has been an explosion of publications on methodology... 

TABLE 2 Summary of Complex Glycan Microarrays Reported in the Literature... 

IDENTIFICATION OF VIRUS RECEPTORS WITH GLYCAN MICROARRAYS... 

FIGURE 3 Examples of virus receptors identified by glycan microarrays. Examples of influenza virus A glycan receptors identified from HMG shotgun array (A), and MVM receptors identified from CFG microarray (B), and the HMG SGM (C). Common motifs for virus recognition are highlighted by boxes. 

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