Microarray gene expression experiments replication

Pan W, Lin J, Le G. 2002. How many replicates of arrays are required to detect gene expression changes in microarray experiments A mixture model approach. Genome Biology 3(5) research0022.1. 

Dudoit S, Yang YH, Callow MJ, Speed TP. 2002. Statistical methods for identifying differentially expressed genes in replicate cDNA microarray experiments. Statistica Sinica 12 111. 

Pan W. 2002. A comparative review of statistical methods for discovering differentially expressed genes in replicated microarray experiments. Bioinformatics 18 546. 

In most microarray experiments, the main aim is to detect differentially expressed genes between two or more samples. Unfortunately, this is an area where the use of distinct analyses still has to provide a concrete method of choice. The best option is to use a statistical test. However, the degree of replication needed for most statistical tests is prohibitive for most microarray experiments. 

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