Microarray cell-signaling

Kopf, E., Shnitzer, D., Zharhary, D. (2005). Panorama Ab Microarray Cell Signaling kit a unique tool for protein expression analysis. Proteomics, 5, 2412-2416. 

Genomic tools 31 This method takes advantage of select genomic (microarray-or PCR-based approaches) and proteomic (antibody array analysis) tools to identify the receptors for growth factors, hormones, cytokines, and other components of cell signaling pathways expressed by a culture of interest. 

Waters KM, Shankaran H, Wiley HS, Resat H, Thrall BD. Integration of microarray and proteomics data for biological pathway analysis and network modeling of epidermal growth factor signaling in human mammary epithelial cells. Keystone Symposia, 2005. 

Expression analysis using DNA microarrays analyzes only the transcriptome it should be mentioned that mRNA abundance in a cell often correlates poorly with the amount of protein synthesized (27). Important regulation takes place at the levels of translation and enzymatic activities. The only effect of a signal transduction pathway that is observed in a gene expression experiment is that at the endpoint of a given pathway. DNA microarrays currently have little value in determining post-translational modifications, which influence the diversity, affinity, function, cellular abundance, and transport of proteins. 

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