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Scanning microscopes

Figure Bl.18.11. Confocal scanning microscope in reflection the pinliole in front of the detector is in a conjugate position to the illumination pinliole. This arrangement allows the object to be optically sectioned. The lens is used to focus the light beam onto the sample and onto the pinliole. Thus, the resulting point spread fimctioii is sharpened and the resolution increased. Figure Bl.18.11. Confocal scanning microscope in reflection the pinliole in front of the detector is in a conjugate position to the illumination pinliole. This arrangement allows the object to be optically sectioned. The lens is used to focus the light beam onto the sample and onto the pinliole. Thus, the resulting point spread fimctioii is sharpened and the resolution increased.
Wolleschensky R, Feurer T, Sauerbrey R and Simon U 1998 Characterization and optimization of a laser-scanning microscope in the femtosecond regime Appl. Phys. B 67 87-94... [Pg.1676]

Vacha M, Yokoyama N, Tokizaki T, Furuki M and Tani T 1999 Laser scanning microscope for low temperature single molecule and microscale spectroscopy based on gradient index optics Rev. Sc/. Instrum. 70 2041-5... [Pg.2505]

JFSM-30 Field Emission Scanning Microscope , JEOL News 12e (1), 29-31 (1974) 60) T. [Pg.148]

Probe storage may be one of the unusual methods of the proposed recording since it does not involve the use of disks at all. Rather, probe storage technology could be implemented in something like the size of a t3q>ical semiconductor chip. It works like a scanning microscope, except that there is an array of these microscopes or probes that reads and writes the data. Each probe addresses an array of bits of information, and the probes write and read in parallel. Several media can-... [Pg.234]

Chaparro AM, Salvador P, Mir A (1996) The scanning microscope for semiconductor characterization (SMSC) Study of the influence of surface morphology on the photoelectrochemical behavior of an n-MoSe2 single crystal electrode by photocurrent and electrolyte electroreflectance imaging. J Electroanal Chem 418 175-183... [Pg.299]

Spengler and Hubert (2002) describe a confocal laser scanning microscope used in conjunction with a TOF mass spectrometer, and also possessing ion imaging... [Pg.61]

Figure 4. DDC (A), serotonin (B), and tyrosine hydroxylase (C) immunore-activity in the posterior region of a wild-type Drosophila ventral ganglion. Tyrosine hydroxylase (TH) encodes the rate-limiting step in dopamine biosynthesis and is a marker for dopamine cells. B and C are the same CNS assayed for both serotonin and TH. M, medial dopamine neurons VL, ventrolateral serotonin neurons DL, dorsolateral dopamine neurons. Short unmarked arrows in C show vacuolated cells that do not contain DDC immunoreactivity. The immunoreactivity in these cells may represent a nonspecific cross-reactivity of the rat TH antibody. The length bar in A is 50 pM. The images are confocal projections generated on a Molecular Dynamics-2000 confocal laser scanning microscope. Figure 4. DDC (A), serotonin (B), and tyrosine hydroxylase (C) immunore-activity in the posterior region of a wild-type Drosophila ventral ganglion. Tyrosine hydroxylase (TH) encodes the rate-limiting step in dopamine biosynthesis and is a marker for dopamine cells. B and C are the same CNS assayed for both serotonin and TH. M, medial dopamine neurons VL, ventrolateral serotonin neurons DL, dorsolateral dopamine neurons. Short unmarked arrows in C show vacuolated cells that do not contain DDC immunoreactivity. The immunoreactivity in these cells may represent a nonspecific cross-reactivity of the rat TH antibody. The length bar in A is 50 pM. The images are confocal projections generated on a Molecular Dynamics-2000 confocal laser scanning microscope.
Szabo, G., Pine, P., Weaver, J., Kasari, M. and Aszalos, A. (1992). Epitope mapping by photobleaching fluorescence resonance energy transfer measurements using a laser scanning microscope system. Biophys. J. 61, 661-70. [Pg.70]

Lifetime imaging can be implemented both in wide field and in scanning microscopes such as confocal microscopes and two-photon excitation microscopes. The most common implementations in time-domain fluorescence lifetime imaging microscopy (FLIM) are based on TCSPC [8, 9] and time-gating (TG) [2, 10],... [Pg.110]

Implementation of time domain FLIM methods is comparatively straightforward in laser scanning microscopes (LSMs). Here, pointscanning is used so that single channel lifetime detection suffices. In principle, standard fluorescence lifetime detection equipment developed for spectroscopy can be used in combination with point-scanning systems and a pulsed laser. [Pg.117]

At present dedicated TCSPC FLIM boards are commercially available. They are compatible with most LSMS and are easily synchronized with the scanning microscope and pulsed laser. These boards, often plug-in cards for PCs, have a lower deadtime than do the conventional TCSPC electronics intended for use in spectroscopy and the memory bottle neck of the histogram-ming memory has been removed [21, 22], Consequently, these dedicated boards provide higher acquisition speeds. [Pg.117]

Dedicated TCSPC electronics is used in all practical TCSPC-FLIM implementations [21, 22]. There are several issues that should be noted. First of all, the lifetime acquisition has to be synchronized with the scanning of the confocal or multiphoton microscope. To this end, the pixel clock and often the line and frame synchronization signals of the scanning microscope are used. [Pg.117]

Historically, this has been the most constrained parameter, particularly for confocal laser scanning microscopes that require spatially coherent sources and so have been typically limited to a few discrete excitation wavelengths, traditionally obtained from gas lasers. Convenient tunable continuous wave (c.w.) excitation for wide-held microscopy was widely available from filtered lamp sources but, for time domain FLIM, the only ultrafast light sources covering the visible spectrum were c.w. mode-locked dye lasers before the advent of ultrafast Ti Sapphire lasers. [Pg.158]

Fig. 9 Surface modification of cells with ssDNA-PEG-lipid. (a) Real-time monitoring of PEG-lipid incorporation into a supported lipid membrane by SPR. (r) A suspension of small unilamellar vesicles (SUV) of egg yolk lecithin (70 pg/mL) was applied to a CH3-SAM surface. A PEG-lipid solution (100 pg/mL) was then applied, (ii) Three types of PEG-lipids were compared PEG-DMPE (C14), PEG-DPPE (C16), and PEG-DSPE (C18) with acyl chains of 14, 16, and 18 carbons, respectively, (b) Confocal laser scanning microscopic image of an CCRF-CEM cell displays immobilized FITC-oligo(dA)2o hybridized to membrane-incorporated oligo(dT)20-PEG-lipid. (c) SPR sensorigrams of interaction between oligo(dA)2o-urokinase and the oligo (dT)2o-PEG-lipid incorporated into the cell surface, (i) BSA solution was applied to block nonspecific sites on the oligo(dT)20-incorporated substrate, (ii) Oligo(dA)20-urokinase (solid line) or oligo(dT)20-urokinase (dotted line) was applied... Fig. 9 Surface modification of cells with ssDNA-PEG-lipid. (a) Real-time monitoring of PEG-lipid incorporation into a supported lipid membrane by SPR. (r) A suspension of small unilamellar vesicles (SUV) of egg yolk lecithin (70 pg/mL) was applied to a CH3-SAM surface. A PEG-lipid solution (100 pg/mL) was then applied, (ii) Three types of PEG-lipids were compared PEG-DMPE (C14), PEG-DPPE (C16), and PEG-DSPE (C18) with acyl chains of 14, 16, and 18 carbons, respectively, (b) Confocal laser scanning microscopic image of an CCRF-CEM cell displays immobilized FITC-oligo(dA)2o hybridized to membrane-incorporated oligo(dT)20-PEG-lipid. (c) SPR sensorigrams of interaction between oligo(dA)2o-urokinase and the oligo (dT)2o-PEG-lipid incorporated into the cell surface, (i) BSA solution was applied to block nonspecific sites on the oligo(dT)20-incorporated substrate, (ii) Oligo(dA)20-urokinase (solid line) or oligo(dT)20-urokinase (dotted line) was applied...
Fig. 10 Confocal laser scanning microscope images of islets with urokinase (UK) immobilized on the membrane. The green fluorescence indicates positive immunostaining for UK. (a) Islets were modified with oligo(dT)2o-PEG-lipid (C16) or (b) oligo(dT)2o-PEG-lipid (C18) then, oligo (dA)2o-UK was added to the media, (c) Unmodified islets with (left) and without (right) oligo (dT)20-PEG-lipids added to the solution. Insets. Bright field images. Scale bars 100 pm... Fig. 10 Confocal laser scanning microscope images of islets with urokinase (UK) immobilized on the membrane. The green fluorescence indicates positive immunostaining for UK. (a) Islets were modified with oligo(dT)2o-PEG-lipid (C16) or (b) oligo(dT)2o-PEG-lipid (C18) then, oligo (dA)2o-UK was added to the media, (c) Unmodified islets with (left) and without (right) oligo (dT)20-PEG-lipids added to the solution. Insets. Bright field images. Scale bars 100 pm...

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Adhesion in the Scanning Probe Microscope

Analytical methods scanning electron microscope

Applications of Scanning Probe Microscopes (STM, AFM, FFM) to Surface and Colloidal Chemistry

Applications scanning electron microscope

Applications scanning microscope

Artifacts scanning electron microscop

Atomic force microscopy scanning tunnel microscopes

Confocal laser scanning microscop

Confocal laser scanning microscope images

Confocal microscope bilateral laser scanning

Confocal scanning microscope

Confocal scanning microscope image

Electrochemical scanning microscope microelectrode

Electrochemical scanning tunneling microscope

Electron microscopic inspection scanning

Environmental scanning electron microscope ESEM)

Environmental scanning electron microscope ESEM) images

FCS in Laser Scanning Microscopes

Field emission scanning electron microscop

Field emission scanning electron microscope FE-SEM)

Field emission scanning electron microscope analysis

Field emission scanning electron microscopes

Field emission scanning electron microscopes FESEM)

Field emission scanning electron microscopes elements used

Field emission scanning electron microscopes spatial resolution

Field emission scanning electron microscopes studies

Freeze drying scanning electron microscopic

How do Scanning Probe Microscopes Work

Image formation scanning tunneling microscope

Instrumentation scanning confocal microscopes

Laser scanning confocal microscope

Laser scanning confocal microscope LSCM)

Laser scanning electron microscope, resolving

Laser scanning microscopes

Low vacuum scanning electron microscope

Low-temperature scanning tunneling microscopes

Low-voltage scanning electron microscopes

MICRO- AND NANOPATTERNING USING THE SCANNING ELECTROCHEMICAL MICROSCOPE

Magnification scanning electron microscope

Microscope sizing scanning electron

Microscopic studies scanning electronic microscopy

Microscopic studies scanning tunneling microscopy

Microscopic techniques scanning electron microscopy

Microscopy confocal scanning optical microscop

Microstructural imaging in the scanning electron microscope

Most common scanning probe microscope

Multimodal scanning microscope

Multimode scanning probe microscop

Nanotechnology scanning probe microscopes

Near-field scanning microscope

Near-field scanning optical microscope NSOM)

Non-scanning picosecond fluorescence Kerr gate microscope

Optical microscopy confocal scanning microscope

Original scanning tunneling microscope

Particle size determination scanning electron microscop

Phase Morphology Investigation Microscopic Tools, Tips, and Selected Scanning Electron Photomicrographs

Photon scanning-tunneling microscope

Pinhole , confocal scanning-beam laser microscope

Plants scanning electron microscopic studies

Poly scanning electron microscope images

Principle of the Scanning Acoustic Microscope

Resolution scanning electron microscop

STM = scanning tunnelling microscope

Scanning Auger Microscope (SAM)

Scanning Auger microscope

Scanning Hall microscope

Scanning Kelvin probe force microscop

Scanning Kelvin probe force microscope

Scanning Probe Microscopic image

Scanning Thermal Microscope Based on AFM

Scanning Tunneling Microscop

Scanning Tunneling Microscope (STM

Scanning acoustic microscope

Scanning confocal microscopes components

Scanning confocal microscopes designing

Scanning confocal microscopes schematic

Scanning electrochemical microscop

Scanning electrochemical microscope

Scanning electrochemical microscope SECM)

Scanning electrochemical microscope electrolyte solutions

Scanning electrochemical microscope induced

Scanning electron acoustic microscope

Scanning electron microscop

Scanning electron microscop chemical etching

Scanning electron microscop conductive coatings

Scanning electron microscop plasma etching

Scanning electron microscop sample preparation

Scanning electron microscope

Scanning electron microscope (SEM

Scanning electron microscope , evidence investigation

Scanning electron microscope Fig

Scanning electron microscope auger electrons

Scanning electron microscope components

Scanning electron microscope environmental

Scanning electron microscope image

Scanning electron microscope microscopy

Scanning electron microscope of the

Scanning electron microscope photos

Scanning electron microscope process

Scanning electron microscope views

Scanning electron microscope with

Scanning electron microscope with elemental analysis capability

Scanning electron microscope, resolving

Scanning electron microscope, resolving power

Scanning electron microscope/microscopy micrograph

Scanning electron microscopes analysis

Scanning electron microscopic

Scanning electron microscopic , freeze

Scanning electron microscopic analysis

Scanning electron microscopic and

Scanning electron microscopic based

Scanning electron microscopic based automated image analysis

Scanning electron microscopic photographs

Scanning electron microscopic procedure

Scanning electron microscopic studies

Scanning electronic microscope

Scanning force microscop

Scanning force microscope

Scanning force microscope (SFM

Scanning imaging microscope

Scanning ion conductance microscope

Scanning ion microscope

Scanning laser acoustic microscope

Scanning microscopic light scattering

Scanning near-field optical microscop

Scanning near-field optical microscope

Scanning near-field optical microscopes SNOM)

Scanning optical microscope

Scanning photoemission microscop

Scanning potential microscope

Scanning probe microscop

Scanning probe microscope

Scanning probe microscope (SPM

Scanning probe microscope electrochemical techniques

Scanning probe microscope lithography

Scanning probe microscopes force microscopy

Scanning probe microscopic methods

Scanning probe microscopy microscope

Scanning transmission electron microscop

Scanning transmission electron microscope

Scanning transmission electron microscope STEM)

Scanning transmission electron microscope advantages

Scanning transmission electron microscope analyses

Scanning transmission electron microscope analyses small particles

Scanning tunnehng microscope

Scanning tunnel microscope

Scanning tunneling microscope

Scanning tunneling microscope atoms with

Scanning tunneling microscope demonstration

Scanning tunneling microscope electrochemical cell with

Scanning tunneling microscope techniques

Scanning tunneling microscope, advantages

Scanning tunneling microscopic

Scanning tunneling microscopic characterization

Scanning tunneling microscopic image

Scanning tunneling microscopic methods

Scanning tunneling microscopic scans

Scanning tunneling/atomic force microscope

Scanning tunnelling microscope

Scanning tunnelling microscope variable temperature

Scanning tunnelling microscopic

Schematic diagram scanning tunneling microscope

Secondary electron microscope scanning mode

Source scanning electron microscope

Specimen preparation method scanning electron microscop

Surfaces scanning tunnehng microscope

TCSPC laser scanning microscope

Tandem scanning microscope

The Laser Scanning Microscope

The Scanning Transmission Electron Microscope

The Scanning Transmission Electron Microscope (STEM)

The Scanning Tunneling Microscope (STM) Images of Individual Atoms on Surfaces

The scanning tunneling microscope

The scanning tunnelling microscope

Use of scanning electron microscope

Variable pressure scanning electron microscope

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