Confocal scanning microscope image

Fig. 4.7 Confocal scanning microscope image of (top) a spinodal phase separation structure Ti02 thin film, and (bottom) the cross-sectional height profile of the above picture across the solid line. Reproduced with permission from Ref. [100].

J.-A. Conchello, E. W. Hanssen "Enhanced 3-D Re- [117] construction from Confocal Scanning Microscope Images. Deterministic and Maximum Likelihood Recon- [118] strauctions, App. Opt. 29 (1990) no. 26. 3795-3804. 

Fig. 9 Surface modification of cells with ssDNA-PEG-lipid. (a) Real-time monitoring of PEG-lipid incorporation into a supported lipid membrane by SPR. (r) A suspension of small unilamellar vesicles (SUV) of egg yolk lecithin (70 pg/mL) was applied to a CH3-SAM surface. A PEG-lipid solution (100 pg/mL) was then applied, (ii) Three types of PEG-lipids were compared PEG-DMPE (C14), PEG-DPPE (C16), and PEG-DSPE (C18) with acyl chains of 14, 16, and 18 carbons, respectively, (b) Confocal laser scanning microscopic image of an CCRF-CEM cell displays immobilized FITC-oligo(dA)2o hybridized to membrane-incorporated oligo(dT)20-PEG-lipid. (c) SPR sensorigrams of interaction between oligo(dA)2o-urokinase and the oligo (dT)2o-PEG-lipid incorporated into the cell surface, (i) BSA solution was applied to block nonspecific sites on the oligo(dT)20-incorporated substrate, (ii) Oligo(dA)20-urokinase (solid line) or oligo(dT)20-urokinase (dotted line) was applied...

Fig. 10 Confocal laser scanning microscope images of islets with urokinase (UK) immobilized on the membrane. The green fluorescence indicates positive immunostaining for UK. (a) Islets were modified with oligo(dT)2o-PEG-lipid (C16) or (b) oligo(dT)2o-PEG-lipid (C18) then, oligo (dA)2o-UK was added to the media, (c) Unmodified islets with (left) and without (right) oligo (dT)20-PEG-lipids added to the solution. Insets. Bright field images. Scale bars 100 pm...

Fig. 23 Confocal laser scanning microscopic image of rhodamine-labeled SiP coated with PMMA brush The diameter of silica particle core is 230 nm, and the Mn of the graft polymer is 256000...

Figure 4.10 A confocal laser scanning microscope image taken through the dorsal skinfold window chamber on a tie-2 GFP (green fluorescent protein) mouse. Two capillaries in which the endothelium is expressing GFP are shown in the image.

Figure 1. Confocal laser scanning microscope images of K562 cells.

Figure 2. Confocal laser scanning microscopic image (A) fluorescence of pectin, (B) fluorescence of soybean flour protein, (C) fluorescence spectra of pectin and soybean flour protein, and (D) reflection of poly(ethylene oxide). Field width A, B and D, 480 pm.

Figure 13. Confocal laser scanning microscope images of a concentrated suspension using image acquisition at different wavelengths. On the right is a white-light image in which the oil phase is relatively transparent, and on the left is a fluorescent image in which the oil phase is observed and the mineral phase is essentially transparent. All of the features in this image appear in focus because it is made up of the sum of several in-focus planes.

Figure 1. Confocal laser scanning microscope images of leukemic cells a) in a case of primary ALL b) in a case of relapse ALL.

Fig. 6.1 Schematic of a laser confocal scanning microscope. The laser illumination spot is scanned across the sample. Reflected light is de-scanned and passed through the beam splitter and the confocal aperture to the detector. The detector of transmitted light may be present as an accessory, but it gives a normal, not a confocal, optical microscope image.

A full 3D reconstruction gives exact, not statistical, 3D information. Clearly, such a reconstruction requires many images. These may be images of parallel planes, from the TEM of serial sections, or from the AFM of the serial cut faces that microtomy would leave. The confocal scanning microscope (see Section 6.2) is often used to form a 3D image by taking... 

Cork T and Kino G S 1996 Confocal Scanning Optical Microscopy and Related Imaging Systems (New York Academic) Gu Min 1996 Principles of Three Dimensional Imaging In Confocal Microscopes (Singapore World Scientific)... 

Spengler and Hubert (2002) describe a confocal laser scanning microscope used in conjunction with a TOF mass spectrometer, and also possessing ion imaging... 

Figure 4. DDC (A), serotonin (B), and tyrosine hydroxylase (C) immunore-activity in the posterior region of a wild-type Drosophila ventral ganglion. Tyrosine hydroxylase (TH) encodes the rate-limiting step in dopamine biosynthesis and is a marker for dopamine cells. B and C are the same CNS assayed for both serotonin and TH. M, medial dopamine neurons VL, ventrolateral serotonin neurons DL, dorsolateral dopamine neurons. Short unmarked arrows in C show vacuolated cells that do not contain DDC immunoreactivity. The immunoreactivity in these cells may represent a nonspecific cross-reactivity of the rat TH antibody. The length bar in A is 50 pM. The images are confocal projections generated on a Molecular Dynamics-2000 confocal laser scanning microscope.

Lifetime imaging can be implemented both in wide field and in scanning microscopes such as confocal microscopes and two-photon excitation microscopes. The most common implementations in time-domain fluorescence lifetime imaging microscopy (FLIM) are based on TCSPC [8, 9] and time-gating (TG) [2, 10],... 

Fig. 12 CNTs act as a vector for drug delivery into living cells. After incubation of HeLa cells with AlexaFluor594-labeled SWNTs for 12 h at 37 °C, living cells were observed under confocal fluorescence microscope for a CNT uptake study, (a) Images show dual confocal detection of AlexaFluor594-SWNT (red) internalized into cells with the membrane stained by AlexaFluor488 (green), (b) Series of images of different z-focal scanning planes down through cells. (Adapted from [61])...

Figure 7.15 Chemical structure of the 2,6-bis(l methylbenzimidazolyl) pyridine (Mebip) end-capped monomer (24) and optical microscopic images of its resulting MSP gels (8 wt% in acetonitrile) formed with (a) Zn(C104)2 and (b) Zn(C104)2/La(C104)3 (mole ratio = 97 2) obtained using a laser scanning confocal microscope operated in transmitted mode (Weng et al. 2006).

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