Laser scanning confocal microscope LSCM

Of the five probes evaluated only Probes 1, 3, and 5 were worth evaluating in detail, as the others had small Stokes shift (excitation wavelength, /v, minus emission wavelength, /Vln) or no detectable emission. Fluorescence behavior was measured using a Zeiss LSM 510 Laser Scanning Confocal Microscope (LSCM) and an Ocean Optics USB2000 spectrometer. 

These fluctuations also occur when there are temperature or instabilty problems in the Acousto Optical Transmissions Filter (AOTF) of Laser Scanning Confocal Microscopes (LSCM), or when there is incorrect... 

Use of Laser Scanning Confocal Microscopes (LSCM) for FISH Imaging... 

Autofluorescence characteristics of minerals have been described by Henkel (1989). In this chapter, we document the visualization and identification of chalcedony crystals in electric organs, by using a Leica TCS - SP2 Laser Scanning Confocal Microscope (LSCM). This microscope has three ion lasers i.e. argon with emission band in 458 run (cyan), 476 ran (blue-green), 488 nm (green) and 514 nm (yellow) He/Ne with emission band in 543 ran (red) and He/Ne with emission band in 633 nm (blue). Since chalcedony is characteristically an autofluorescent mineral, we were allowed to obtain images of crystals. 

The phase separation kinetics observed in situ by laser-scanning confocal microscope (LSCM) at three different locations (lower, middle, and upper) in the mixture along the irradiation direction (Z) is illustrated in Figure 6.8 for a PSAF/MMA (10/90) mixture under I = 0.03 mW cm . For all locations along the direction of propagation of the UV light, the co-continuous structures appear, then coarsen with... 

Several kinds of microscopic methods which can construct a 3-D image of the solid sample have been developed. Depending on the spatial resolution obtained in the 2-D observation, the reliable size of pores and pore volumes vary to a large extent. Typically, the finest resolution is a few micrometers for X-ray tomography, a few hundred nanometers for laser scanning confocal microscope (LSCM), and below 10 nm for high resolution... 

Epifluorescence microscope and/or laser scanning confocal microscope (LSCM). 

Fig.1 Schematic representing the optical pathway and image information flow of the laser scanning confocal microscopy (LSCM) technique. All principle components of generic LSCM are labeled (Redrawn in part frtnn Carl Zeiss The Confocal Laser Scanning Microscope)...

Figure 1 shows the spatial resolution of various types of microscopes and scattering methods. The figure includes 3D microscopy such as laser scanning confocal microscopy (LSCM), X-ray computerized tomography (X-ray CT), and transmission electron microtomography (TEMT). Although no experimental results obtained from the X-ray CT will be presented in the present review paper, we note that the microscopy [4] is particularly usefiil to... 

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