Confocal microscope bilateral laser scanning

Fig. 2. Diagram of the optical path of the bilateral laser scanning confocal microscope (InSIGHT). Real-time confocal imaging is obtained through the use of a double-sided mirror which simultaneously scans the sample and the oculars or detector at video rates. BSO, beam shaping optics LM, laser mirror D, dichroic M4, mirrors SM, scanning mirror G, galvonometer L1-L3 lenses S, variable slit.

Fig. 3. Simplified diagram of the confocal components of the AC AS 570. The laser beam is expanded to provide a diffraction limited spot at the sample. A focusing lens in front of the detection system directs the fluorescence from the focal plane through an adjustable pinhole. In the bilateral laser scanning confocal microscope, a similar technique is employed with a cursor rather than a point for illumination and detection.

Lily pollen was stained with acridine orange and imaged using the bilateral laser scanning confocal microscope (InSIGHT). Figure 23 shows a three-dimensional reconstruction of 49 separate slices, using a modified version of the SFP... 

Visscher, K., Brakenhoff G.J. and Krol, J.J. (1993) Micromanipulation by multiple optical traps created by a single fast scanning trap integrated with bilateral confocal scanning laser microscope. Cytometry, 14, 105—114. 

---