Scanning confocal microscopes components

Confocal laser scanning microscopy. A laser scanning confocal microscope system was used to analyze the microstructure of the fdm components and films A model IRBE (inverted) microscope (Leica Microsystems, Bannockburn, IL) with a universal stage and 20X objective lens was coupled to a TCS NT/SP scanner head (Leica Microsystems, Exton, PA). Sheets of samples or powders in MatTek dishes (MatTek Corp., Ashland, MA) were illuminated with the 488 nm line of an Argon laser and fluorescence was collected in one channel (520-580 nm) and reflection at 488 nm was collected in another. Fluorescence emission spectra for the sample powders of SFP and Pectin were collected in the range of 500-680 nm. 

Fig. 3. Simplified diagram of the confocal components of the AC AS 570. The laser beam is expanded to provide a diffraction limited spot at the sample. A focusing lens in front of the detection system directs the fluorescence from the focal plane through an adjustable pinhole. In the bilateral laser scanning confocal microscope, a similar technique is employed with a cursor rather than a point for illumination and detection.

Fig.1 Schematic representing the optical pathway and image information flow of the laser scanning confocal microscopy (LSCM) technique. All principle components of generic LSCM are labeled (Redrawn in part frtnn Carl Zeiss The Confocal Laser Scanning Microscope)...

Structural variations may be also produced at the microscopical scale and are able to produce significant improvements in our understanding of stressor effects. Observation of the biofilm architecture and characterisation of the different fractions (i.e. algae, bacteria, mucopolysaccharides) may be useful to identify particular effects of toxicants to selective components of the biofilm. The use of confocal laser scanning microscopy remains promising [25]. 

Skuce, P. J., Johnston, C. F., Fairweather, I., Halton, D. W. and Shaw, C. (1990a) A confocal scanning microscope study of the peptidergic and serotonergic components of the nervous system in larval Schistosoma mansoni. Parasitology 101 227-234. 

Confocal laser scanning microscope A special tool for fluorescence, prevents blurring of images by placing a pinhole at the confocal image plan Location of proteins, lipids, and cellular components ... 

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