Confocal laser scanning microscope images

Fig. 9 Surface modification of cells with ssDNA-PEG-lipid. (a) Real-time monitoring of PEG-lipid incorporation into a supported lipid membrane by SPR. (r) A suspension of small unilamellar vesicles (SUV) of egg yolk lecithin (70 pg/mL) was applied to a CH3-SAM surface. A PEG-lipid solution (100 pg/mL) was then applied, (ii) Three types of PEG-lipids were compared PEG-DMPE (C14), PEG-DPPE (C16), and PEG-DSPE (C18) with acyl chains of 14, 16, and 18 carbons, respectively, (b) Confocal laser scanning microscopic image of an CCRF-CEM cell displays immobilized FITC-oligo(dA)2o hybridized to membrane-incorporated oligo(dT)20-PEG-lipid. (c) SPR sensorigrams of interaction between oligo(dA)2o-urokinase and the oligo (dT)2o-PEG-lipid incorporated into the cell surface, (i) BSA solution was applied to block nonspecific sites on the oligo(dT)20-incorporated substrate, (ii) Oligo(dA)20-urokinase (solid line) or oligo(dT)20-urokinase (dotted line) was applied...

Fig. 10 Confocal laser scanning microscope images of islets with urokinase (UK) immobilized on the membrane. The green fluorescence indicates positive immunostaining for UK. (a) Islets were modified with oligo(dT)2o-PEG-lipid (C16) or (b) oligo(dT)2o-PEG-lipid (C18) then, oligo (dA)2o-UK was added to the media, (c) Unmodified islets with (left) and without (right) oligo (dT)20-PEG-lipids added to the solution. Insets. Bright field images. Scale bars 100 pm...

Fig. 23 Confocal laser scanning microscopic image of rhodamine-labeled SiP coated with PMMA brush The diameter of silica particle core is 230 nm, and the Mn of the graft polymer is 256000...

Figure 4.10 A confocal laser scanning microscope image taken through the dorsal skinfold window chamber on a tie-2 GFP (green fluorescent protein) mouse. Two capillaries in which the endothelium is expressing GFP are shown in the image.

Figure 1. Confocal laser scanning microscope images of K562 cells.

Figure 2. Confocal laser scanning microscopic image (A) fluorescence of pectin, (B) fluorescence of soybean flour protein, (C) fluorescence spectra of pectin and soybean flour protein, and (D) reflection of poly(ethylene oxide). Field width A, B and D, 480 pm.

Figure 13. Confocal laser scanning microscope images of a concentrated suspension using image acquisition at different wavelengths. On the right is a white-light image in which the oil phase is relatively transparent, and on the left is a fluorescent image in which the oil phase is observed and the mineral phase is essentially transparent. All of the features in this image appear in focus because it is made up of the sum of several in-focus planes.

Figure 1. Confocal laser scanning microscope images of leukemic cells a) in a case of primary ALL b) in a case of relapse ALL.

Spengler and Hubert (2002) describe a confocal laser scanning microscope used in conjunction with a TOF mass spectrometer, and also possessing ion imaging... 

Figure 4. DDC (A), serotonin (B), and tyrosine hydroxylase (C) immunore-activity in the posterior region of a wild-type Drosophila ventral ganglion. Tyrosine hydroxylase (TH) encodes the rate-limiting step in dopamine biosynthesis and is a marker for dopamine cells. B and C are the same CNS assayed for both serotonin and TH. M, medial dopamine neurons VL, ventrolateral serotonin neurons DL, dorsolateral dopamine neurons. Short unmarked arrows in C show vacuolated cells that do not contain DDC immunoreactivity. The immunoreactivity in these cells may represent a nonspecific cross-reactivity of the rat TH antibody. The length bar in A is 50 pM. The images are confocal projections generated on a Molecular Dynamics-2000 confocal laser scanning microscope.

Fig. 4.27. Expression of GPP labeled transcription factor in the salivary gland cells of Drosophila [62]. The generation of transcription factor molecules can be seen in the cytoplasmic reticulum as well as the distribution on the polytenic chromosomes in the nucleus. Image taken in a confocal laser scan microscope from C. Zeiss, modified for APD Imaging [6f]...

Figure 8. Microscopic images of the fractural surfaces of PEO-included composite samples obtained by scanning electron microscope (left), laser microscope (middle), and confocal laser scanning microscope in confocal fluorescence and confocal reflection two channels (right). Field width 520 pm (left) and 480 pm (middle and right).

E.P. Buurman, R. Sanders, A. Draaijer, H.C. Gerritsen, J.J.F. van Veen, P.M. Houpt, Y.K. Levine, Fluorescence lifetime imaging using a confocal laser scanning microscope, Scanning, 14, 155-159 (1992)... 

Microscope equipped with differential interference contrast (DIC) and/or fluorescence imaging capabilities. We use either a Nikon Eclipse TE300 inverted microscope equipped with epifluorescent illumination or a Fluoview FVIOOO FVIO-ASW confocal laser scanning microscope, both equipped with 20x and 60x objectives. 

Inverted confocal laser scanning microscope, Fluoview FVIOOO (Olympus Corp.). The microscope is equipped with an additional, separate scanning unit to move a 405- nm laser (25 mW, FV5-LD405, Olympus Corp., reeNote 5) independent of the imaging lasers inside the field of view see Note 6). 

Images were taken with a confocal laser scanning microscope (Tme Confocal Scanner 4D, Leica, Heidelbeig, Germany) equipped with an aigon-krypton laser and coupled to a Leitz DM IRB inverted microscope (Leica).Excitation was at 488 nm, and emission was band-pass filtered at 515nm. 

Confocal laser scanning microscope A special tool for fluorescence, prevents blurring of images by placing a pinhole at the confocal image plan Location of proteins, lipids, and cellular components ... 

Confocal images were obtained by Leica Confocal Laser Scanning Microscope TCS SR For capsules visualization 100 x oil inunersion objective was used throughout. 10 pi of the SNARF-1 dextran/urease capsules suspension was placed on a covershp. To this suspension 10 pi of 0.1 mol/L urea is added. After about 20 min confocal images were obtained. The red fluorescence emission was accumulated at 600-680 mn after excitation by the FITC-TRIC-TRANS laser at 543 mn. 

Fig.1 Schematic representing the optical pathway and image information flow of the laser scanning confocal microscopy (LSCM) technique. All principle components of generic LSCM are labeled (Redrawn in part frtnn Carl Zeiss The Confocal Laser Scanning Microscope)...

Figure 24.4 Confocal laser scanning microscope [CLSM] images of iiving influenza A/PR/8-infected MDCK cells stained with (a) NA mRNA specific PNA FiT probe 1 or (b) Ml mRNA specific PNA FiT probe 2. At 5h post infection ceiis were measured in the TO channei (Ex = 488nm,...

Visualization and documentation of the results The slides are then dried and moimted. Observation of the hybridization signals can be performed by a conventional epifluorescent microscope or by a confocal laser scanning microscope (CLSM). CLSM is particularly recommended in the case of thick sections, or samples with high fluorescence backgroimd or if fiu"-ther image analysis (quantification, 3D-structure) is required. 

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