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Mutagenesis

Stability and Purification of the Recombinant Protein. There are no hard and fast rules specifying, eg, whether a recombinant protein is available in a soluble state in the cell. In some cases, the expression system must be engineered by in vitro mutagenesis to optimize overall yield of the protein. [Pg.237]

Fig. 9. Mutagenesis by a synthetic oligonucleotide of a cloned sequence available in single-stranded form (a) single-stranded M13 template containing uracil (U) residues (b) double-stranded product, uracil residues are not mutagenic (c) strong selection for M13 phages containing mutation of interest (23). Fig. 9. Mutagenesis by a synthetic oligonucleotide of a cloned sequence available in single-stranded form (a) single-stranded M13 template containing uracil (U) residues (b) double-stranded product, uracil residues are not mutagenic (c) strong selection for M13 phages containing mutation of interest (23).
Linker-Scanning Mutagenesis. Using linker-scanning mutagenesis (24) small sequences of DNA are removed and replaced with a synthetic restriction fragment or linker. This technique is commonly used in analysis of promoters and other control sequences in DNA, while preserving the spatial relationship between the sequences. [Pg.237]

Sequence analysis and mutagenesis experiments indicated that the thiolate ligand is provided by a-Cys-275 (163—165). These predictions have been... [Pg.89]

Fig. 6. Polymerase chain reaction (PCR) mediated site-directed mutagenesis. The 5 and 3 ends of the nucleotide strands are indicated. The four arrows surrounding the DNA template represent oligonucleotide primers 1—4. See text for discussion. Fig. 6. Polymerase chain reaction (PCR) mediated site-directed mutagenesis. The 5 and 3 ends of the nucleotide strands are indicated. The four arrows surrounding the DNA template represent oligonucleotide primers 1—4. See text for discussion.
En me Mechanism. Staphylococcal nuclease (SNase) accelerates the hydrolysis of phosphodiester bonds in nucleic acids (qv) some 10 -fold over the uncatalyzed rate (r93 and references therein). Mutagenesis studies in which Glu43 has been replaced by Asp or Gin have shown Glu to be important for high catalytic activity. The enzyme mechanism is thought to involve base catalysis in which Glu43 acts as a general base and activates a water molecule that attacks the phosphodiester backbone of DNA. To study this mechanistic possibiUty further, Glu was replaced by two unnatural amino acids. [Pg.206]

BioRad product bulletin, Lit-245 0890 90-0910a, Muta-Gene M13 In Vitro Mutagenesis Kit, Cat. No. 170-3580, BioRad Laboratories, Hercules, Ca., 1990. [Pg.207]

E. Vogel and G. Rohrbom, eds.. Chemical Mutagenesis in Mammals and Man, Spriuger-Vedag, Berlin, 1970. [Pg.240]

A. W. Hsie, J. P. O NeiU, and V. K. McEUieney, eds.. Mammalian Cell Mutagenesis The Maturation of Test Systems, Banbury Report No. 2, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1979. [Pg.240]

Live attenuated vaccines for RSV are also being developed. Most of these vaccine candidates are derived from cold adaptation, by passing the vims at progressingly lower temperatures than human body temperatures. However, other means of mutagenesis have been used to generate vaccine candidates (72). Several clinical trials of these vaccines are also in progress (73,74). [Pg.359]


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Acetylcholine receptors directed mutagenesis

Acid-base catalysis, site-directed mutagenesis

Adenosine site-directed mutagenesis

Alanine-scanning mutagenesis

Alanine-scanning mutagenesis protein

Aminotransferases mutagenesis

Antioxidant response and mutagenesis

Arsenic mutagenesis

Assay microbial mutagenesis

Assay, mutagenesis

Bacteria chemical mutagenesis

Bacterial mutagenesis

Bacteriorhodopsin site-directed mutagenesis

Bacteriorhodopsins site-directed mutagenesis

Calculation of Mutagenesis Hot-Spots

Carboxypeptidase site-directed mutagenesis

Carcinogenesis and mutagenesis

Carcinogenesis, Mutagenesis, and Teratogenesis

Cassette mutagenesis

Chemical Modification and Site-Directed Mutagenesis

Chemical mutagenesis 42-46 literature

Chemical mutagenesis complexity

Chemical mutagenesis radiation

Chemically-Induced Mutagenesis

Chemokines mutagenesis study

Chimeragenesis and site-directed mutagenesis

Chorismate mutase mutagenesis

Classical mutagenesis

Codon-level mutagenesis

Combinatorial multiple-cassette mutagenesis

Combinatorial multiple-cassette mutagenesis CMCM)

Combinatorial saturation mutagenesis

Combining Site-directed Mutagenesis with Chemical Modification

Computational mutagenesis

Concepts of Mutagenesis

Control of Translesion Synthesis and Mutagenesis

Cysteine mutagenesis

Cysteine using site-directed mutagenesis

Cytochrome P450 reductase mutagenesis

Cytochrome mutagenesis

DNA mutagenesis

Deletion mutagenesis

Dioxygenase site-directed mutagenesis

Directed cassette mutagenesis

Directed evolution gene mutagenesis methods

Directed random mutagenesis

Directed saturation mutagenesis

Dopamine site-directed mutagenesis

ENU-mutagenesis

Environmental Mutagenesis Branch

Environmental mutagenesis

Environmental mutagenesis toxicology

Enzymes mutagenesis methods

Epitope scanning mutagenesis

Factors in Metal Mutagenesis

FeMoco site-directed mutagenesis

Flavocytochrome site-directed mutagenesis

Focused mutagenesis

Formaldehyde induced mutagenesis

Frameshift mutagenesis

Gene mutagenesis methods

Gene-site saturation mutagenesis

Genetic toxicity mutagenesis

Heme mutagenesis studies

Hepatocyte-Mediated Mutagenesis of Rat Liver Epithelial Lines

History of Research on Chemical Mutagenesis

Homolog-scanning mutagenesis

Human population mutagenesis

In vitro mutagenesis

Induced mutagenesis

Insertion mutagenesis

Insertional mutagenesis

Ionizing radiation mutagenesis induced

Iron proteins site-directed mutagenesis

Iron-sulfur clusters site-directed mutagenesis

Iterative saturated mutagenesis

Iterative saturation mutagenesis

Kinase mutagenesis studies

Lesions mutagenesis induced

Liver cultures mutagenesis

Lysozyme enzyme, mutagenesis

Lysozyme mutagenesis

Lysozyme site-directed mutagenesis

Mammals mutagenesis

Mechanisms of DNA Damage-Induced Mutagenesis

Modulation of mutagenesis

Mouse lymphoma mutagenesis

Multiple cassette mutagenesis

Mutagenesis Amino Acid

Mutagenesis DNA shuffling

Mutagenesis Escherichia coli

Mutagenesis Rossman

Mutagenesis analysis

Mutagenesis and DNA shuffling

Mutagenesis and Errors in Transcription

Mutagenesis and Its Literature

Mutagenesis and PCR

Mutagenesis application

Mutagenesis assay, Salmonella

Mutagenesis assay, Salmonella typhimurium

Mutagenesis assays, applications

Mutagenesis causes

Mutagenesis chemical

Mutagenesis combinatorial

Mutagenesis combinatorial methods

Mutagenesis cysteine scanning

Mutagenesis damage

Mutagenesis definition

Mutagenesis directed evolution

Mutagenesis dose-response curves

Mutagenesis epPCR)

Mutagenesis error-prone polymerase chain reaction

Mutagenesis experimental studies

Mutagenesis gene shuffling

Mutagenesis in mammals

Mutagenesis induction by xenobiotics

Mutagenesis mammalian

Mutagenesis mammalian assays

Mutagenesis mammalian cell culture tests

Mutagenesis metabolism

Mutagenesis metal compounds

Mutagenesis method

Mutagenesis microbial tests

Mutagenesis oligonucleotide-directed

Mutagenesis oxidative

Mutagenesis oxidative reactions

Mutagenesis peroxidase

Mutagenesis point

Mutagenesis polymerase chain reaction

Mutagenesis protein folding

Mutagenesis rational

Mutagenesis repair-mediated

Mutagenesis saturation

Mutagenesis scan

Mutagenesis screens

Mutagenesis shuffling

Mutagenesis site-specific

Mutagenesis site-specific, lysozyme

Mutagenesis specificity

Mutagenesis strategies

Mutagenesis study

Mutagenesis test, Salmonella

Mutagenesis thermal stability

Mutagenesis virtual

Mutagenesis, artificial

Mutagenesis, carcinogen risk assessment

Mutagenesis, ligand binding

Mutagenesis, protein function analysis

Mutagens mutagenesis

Mutation and mutagenesis

Mutational approaches insertional mutagenesis

Mutational approaches site-directed mutagenesis

Mutations/mutagenesis

Natural Mutagenesis

Natural evolution mutagenesis methods

Nucleic acids site-directed mutagenesis

OECD and ICH of mutagenesis test

Oligonucleotide cassette mutagenesis

Oligonucleotide directed codon mutagenesis

Oligonucleotide-mediated mutagenesi

Origin of Mutagenesis

PCR mutagenesis

Pesticide mutagenesis evaluation

Phage display random mutagenesis

Polymerase chain reaction , random mutagenesis method

Polymerase chain reaction site directed mutagenesis

Polymerase chain reaction site-directed mutagenesis based

Polymerase chain reaction site-specific mutagenesis

Protein folding site-directed mutagenesis

Protein mutagenesis

Protein site-directed mutagenesis

Proteins stability site-directed mutagenesis

Random mutagenesis

Random point mutagenesis

Receptor characterization site directed mutagenesis

Receptor mutagenesis

Retroviral mutagenesis

Ribonuclease mutagenesis

Saccharomyces cerevisiae mutagenesis

Salmonella typhimurium mutagenesis

Scanning mutagenesis

Semi-random mutagenesis

Sequence saturation mutagenesis

Sequence-directed mutagenesis

Side-directed mutagenesis

Signal mutagenesis

Site directed mutagenesis mutation

Site directed mutagenesis protein stability/engineering

Site saturation mutagenesis

Site-Directed Mutagenesis Substituting Individual Amino Acids in Proteins

Site-Directed Mutagenesis in the Study of Substrate Selectivity and Electron Transfer

Site-directed mutagenesi

Site-directed mutagenesis

Site-directed mutagenesis activity effects

Site-directed mutagenesis chemical modification with

Site-directed mutagenesis complex

Site-directed mutagenesis conformation

Site-directed mutagenesis crystallization

Site-directed mutagenesis data collection

Site-directed mutagenesis effects

Site-directed mutagenesis enzyme engineering

Site-directed mutagenesis enzyme modification

Site-directed mutagenesis enzyme structure

Site-directed mutagenesis experiments

Site-directed mutagenesis hydrogen bonding

Site-directed mutagenesis implications for catalysis

Site-directed mutagenesis nuclear magnetic resonance

Site-directed mutagenesis of EF-Tu

Site-directed mutagenesis of enzyme

Site-directed mutagenesis protease

Site-directed mutagenesis refinement

Site-directed mutagenesis steady-state kinetics

Site-directed mutagenesis structural analysis

Site-directed mutagenesis studies

Site-directed mutagenesis, catalytic antibodies

Site-directed mutagenesis, serotonin

Site-directed mutagenesis, serotonin receptors

Site-directed mutagenesis. See

Site-selective mutagenesis

Site-specific mutagenesis principle

Site-specific mutagenesis restriction—selection

Sources and Types of Chemical Mutagenesis Literature

Specificity site directed mutagenesis

Spontaneous mutagenesis

Stochastic Description of Mutagenesis and Killing

Strains random mutagenesis

Structure-activity correlations mutagenesis

Structure-guided mutagenesis

Subject mutagenesis

Subtilisin site-directed mutagenesis

T-DNA insertional mutagenesis

Targeted mutagenesis

Targeted random mutagenesis

Techniques for Monitoring and Assessing the Significance of Mutagenesis in Human Populations

The Literature of Chemical Mutagenesis

The Pol Mutagenesis Pathway in Eukaryotes

The State of Chemical Mutagenesis Literature

Thrombin site-directed mutagenesis

Tn5 mutagenesis

Toxicity Mutagenesis studies

Transposon mutagenesis

UV mutagenesis

Ultraviolet light mutagenesis induced

Variation of Enzyme Structure Site-directed Mutagenesis

Xylose site-directed mutagenesis

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