Tn5 mutagenesis

Using Tn5 mutagenesis, complementation analysis, and DNA-sequencing experiments, a gene designated lag-1 (lipopolysaccharide-associated gene)... 

Vanstockem,M., Michiels, K., Vanderleyden, J. and Van Gool, A., 1987 Transposon mutagenesis of Azospirillum hrasilense and Azospirillum lipoferum, physical analysis of Tn5 and lr 5-moh insertion mutants. Appl. Environ. Microbiol. 53 410-415. 

For genetic studies in bacteria that are not easily manipulated, a trans-poson capable of random mutagenesis and tagging of mutated genes is extremely valuable. Random mutagenesis of D. desulfuricans G20 was documented when derivatives of Tn5 that have increased expression of trans-posase (Simon 1984 Wolk et al. 1991) were used (Wall et al. 1996). 

Petzke L, Luzhetskyy A (2009) In vivo Tn5-based Transposon Mutagenesis of Streptomy-cetes. Appl Microbiol Biotechnol 83 979... 

A mutant defective in the chemotaxis toward 4-hydroxybenzoate in P. putida was obtained by Tn5 transposon mutagenesis, and allowed identification of a gene cluster pcaRKF that encodes enzymes of the 3,4-dihydroxybenzoate branch of the (i-ketoadi pate pathway pcaR encodes regulatory genes, pcaK the thiolase in the last step of the pathway, and pcaF is involved in transport of, and chemotaxis toward 4-hydroxybenzoate (Harwood et al. 1994). 

Mini-Tn5 and TniO derivatives Gene insertion mutagenesis, promoter probing, chromosomal insertion of cloned DNA [57-60]... 

Petzke, L. and Luzhetskyy, A. (2009) In vivo Tn5-based transposon mutagenesis of Streptomycetes. Appl. Microbiol. Biotechnol, 83 (5), 979-986. 

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