Saccharomyces cerevisiae mutagenesis

Ferguson, L. R. (1985) Petite mutagenesis in Saccharomyces cerevisiae strain D5. Prog. Mutat. Res., 5, 229-234... 

Polakowska, R. Putrament, A. (1979) Mitochondrial mutagenesis in Saccharomyces cerevisiae... 

Hemrika, W., R. Renirie, S. Macedo-Ribeiro, A. Messershmidt, and R. Wever. 1999. Heterologous expression of the vanadium-containing chloroperoxidase from Curvu-laria inaequalis in Saccharomyces cerevisiae and site-directed mutagenesis of the active site residues His(496), Lys(353), Arg(360), and Arg(490). J. Biol. Chem. 274 23820-23827. 

Copper ligands of the recombinant tyrosinase from A. oryzae expressed in Saccharomyces cerevisiae or Escherichia coli were identified by site-directed mutagenesis [145],... 

Sora S, Carbone MLA, Pacciatini M, et al. 1986. Disomic and diploid meiotic products induced in Saccharomyces cerevisiae by the salts of 27 elements. Mutagenesis 1 21 -28. 

Phan, L. D Perentesis, J. P Bodley, J. W. (1993). Saccharomyces cerevisiae elongation factor 2. Mutagenesis of the histidine precursor of diphthamide yields a functional protein that is resistant to diphtheria toxin. J. Biol. Chem. 268, 8665-8668. 

An analysis of mutable sites in the DNA-binding domain of human p53 has been performed using a yeast functional assay. This assay is based on the expression of human p53 introduced into Saccharomyces cerevisiae containing a p53-dependent reporter gene. Random mutagenesis has revealed that mutation at 542 sites in a 1079-bp fragment could inactivate p53 DNA binding. This number is remarkably close to the number of observed sites at... 

Saccharomyces cerevisiae played an important role in the genetic analysis of riboflavin biosynthesis. Mating analysis between riboflavin auxotrophic mutants obtained by random mutagenesis and screening of haploid... 

Mutagenesis studies with Saccharomyces cerevisiae revealed several target mutations within two relatively short interhelical regions of the cytochrome b gene associated with a reduction in sensitivity to Qol fungicides many of which result in impaired respiration and, hence, reduced htness [3-5],... 

Holbeck, S. L., and Strathern, J. N. (1997). A role for REV3 in mutagenesis during double-strand break repair in Saccharomyces cerevisiae. Genetics 147, 1017-1024. 

Rattray, A. J., Shafer, B. K., McGill, G. B., and Strathern,J. N. (2002). The roles of REV3 and RAD57 in double-strand-break-repair-induced-mutagenesis of Saccharomyces cerevisiae. Genetics 162, 1063—1077. 

Torpey, L. E., Gibbs, P. E. M., Nelson, J. R., and Lawrence, C. W. (1994). Cloning and sequence of REV7, a gene whose function is required for DNA damage-induced mutagenesis in Saccharomyces cerevisiae. Yeast 10, 1503-1509. 

Lawrence, G. W. (1982). Mutagenesis in Saccharomyces cerevisiae. Adv. Genet. 21, 173-254. Li, Z., Xiao, W., McCormick, J.J., and Maher, V. M. (2002). Identification of a protein essential for a major pathway used by human cells to avoid UVAnduced DNA damage. Proc. Natl. Acad. Sci. USA 99, 4459-4464. 

A mutant strain of Saccharomyces cerevisiae (DlO-ERl) has been isolated after a two-step mutagenesis of strain 4059-358D SUCl) using ethyl methane... 

Alcalde, M. (2010) Mutagenesis protocols in Saccharomyces cerevisiae by in vivo overlap extension. Methods Mol. Biol., 634, 3-14. 

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