Mutagenesis Escherichia coli

B. 13. Other effects - Mutagenesis (Escherichia coli - reverse mutation assay)... 

Touati D, M Jacques, B Tardat, L Bouchard, S Despied (1995) Lethal oxidative damage and mutagenesis are generated by iron Afur mutants of Escherichia coli protective role of superoxide dismutase. J Bacterial 111 2305-2314. 

Prieto-Alamo, M.J., Abril, N. and Pueyo, C. (1993). Mutagenesis in Escherichia coli K-12 mutants defective in superoxide dismutase or catalase. Carcinogenesis 14, 237-244. 

Induced mutagenesis in Escherichia coli is an active process involving proteins with DNA replication, repair, and recombination functions. The available evidence suggests that mutations are generated at sites where DNA has been damaged and that they arise via an error-prone repair activity. In an attempt to understand what specific contributions to mutagenesis are made by DNA lesions, we have studied the mutational specificity of some carcinogens, such as benzo[a]pyrene and aflatoxin, whose chemical reactions with DNA are... 

In this chapter I will review some aspects of mutagenesis mechanisms and the mutational consequences of DNA damage generated by ben-zo[a]pyrene. The focus will be on knowledge derived from investigations involving the bacterium Escherichia coli. 

Zecherle, G.N. (1990) The ribosomal location and conformation of Escherichia coli protein L7/L12 studied by cysteine site directed mutagenesis and crosslinking. Doctoral Dissertation. University of California at Davis. 

Walker, G.C. and Dobson, P.P. (1979). Mutagenesis and repair deficiencies of Escherichia coli umu C mutants are suppressed by the plasmid pKMIOl. Proc. Gen. Genet. 172 17-24. 

Wilcox, R, Naidoo, A., Wedd, D.J. and Gatehouse, D.G. (1990). Comparison of Salmonella Typhimurium TA102 with Escherichia coli WP2 tester strains. Mutagenesis 5 285-291. 

Yanofsky, C. (1971). Mutagenesis studies with Escherichia coli mutants with known amino acid and base-pair) changes. In Chemical Mutagens, Principles and Methods for Their Detection (Hollaender A., Ed.). Vol. 1, Plenum Press, New York, pp. 283-287. 

A. Dubus, S. Normarj, M. Kania, M. G. P. Page, The Role of Tyrosine 150 in Catalysis of /3-Lactam Hydrolysis by AmpC /3-Lactamase from Escherichia coli Investigated by Site-Directed Mutagenesis , Biochemistry 1994, 33, 8577-8586. 

The production of chromosomal abnormalities by ozone was first observed in plants by Fetner, who noted abnormal anaphases in the root tips of Vicia fava seeds exposed to ozone. Bacterial mutagenesis by ozone has been reported by a number of investigators. Hamelin and Chung noted an increased mutation rate in Escherichia coli exposed to ozone at as low as 0.05 ppm for 5 min and observed that the mutants were sensitive to ozone and X irradiation. They suggested that ozone might interact in DNA repair processes. Other studies indicating ozone-induced... 

Davis, 1. Microbiologic Studies with Ozone. Mutagenesis of Ozone for Escherichia coli. Brooks Air Force Base, Texas School of Aerospace Medicine. USAF Aerospace Medical Center (ATC), June 1%1. 9 pp. 

Selected entries from Methods in Enzymology [vol, page(s)] Equilibrium isotope exchange study of kinetic mechanism, 249, 466 site-directed mutagenesis of Escherichia coli enzyme, 249, 93 positional isotope exchange studies, 249, 423 product inhibition studies of three substrates three products reactions, 249, 207-208. 

Antimutagenic activity. Ethanol (70%) extract of the dried aerial parts, on agar plate, was inactive on Escherichia coli PQ37 vs mitomycin-induced mutagenesis, assessed by the SOS-chromotest method Antioxidant activity. Hexane and methanol extracts of the dried seed, tested on lard at a concentration of 0.06 %, were inactive Seed oil, at undiluted concentration, was active " . Acetone extract of the seed, at a concentration of 0.2 mg/kg, was active. Linoleic acid was used as a substrate in this test " . 

Rodriguez H, Loechler EL. 1993. Mutational specificity of the (+) - anti-diol epoxide of benzo [a] pyrine in a supF gene of an Escherichia coli plasmid DNA sequence context influences hotspots, mutagenic specificity and the extent of SOS enhancements of mutagenesis. Carcinogenesis 14(3) 373-383. 

Kanamaru, K. Aiba, H. Mizuno, T. Transmembrane signal transduction and osmoregulation in Escherichia coli I. Analysis by site-directed mutagenesis of the amino acid residues involved in phosphotransfer between the two regulatory components, EnvZ and OmpR. J. Biochem., 108, 483-487 (1990)... 

Abril, N. Pueyo, C. (1990) Mutagenesis in Escherichia coli lacking catalase. Environ, mol. Mutag., 15, 184-189... 

Kobori, T., H. Sasaki, W. C. Lee, S. Zenno, K. Saigo, M. E. P. Murphy, and M, Tanokura, Structure and site-directed mutagenesis of a flavoprotein from Escherichia coli that reduces nitrocompounds , J. Biol. Chem., 276, 2816-2823 (2001). 

Cloning in Systems Other than Escherichia coli Site-Directed Mutagenesis Permits the Restructuring of Existing Genes... 

Rossman TG, Molina M. 1986. The genetic toxicology of metal compounds II. Enchancement of ultraviolet light-induced mutagenesis in Escherichia coli WP2. Environ Mutagen 8 263-271. 

J. J. Onuffer and J. F. Kirsch, Redesign of the substrate specificity of Escherichia cdi aspartate aminotransferase to that of Escherichia coli tyrosine aminotransferase by homology modeling and site-directed mutagenesis, Protein Sci. 1995, 4, 1750-1757. 

LA Ivanoff, T Towatari, J Ray, BD Korant, SR Petteway Jr. Expression and site-specific mutagenesis of the poliovirus 3C protease in Escherichia coli. Proc Natl Acad Sci (USA) 83 5392-5396, 1986. 

In order to obtain further information about the primary specificity of aspergillopepsin I, we constructed an aspergillopepsin I expression system in Escherichia coli, prepared mutants at positions 76 and 78 of aspergillopepsin I by site-directed mutagenesis, and compared then-molecular and enzymatic properties [30], We showed that the nature of the active site flap is important for deciding the Si substrate specificity of aspartic proteinases [30],... 

Copper ligands of the recombinant tyrosinase from A. oryzae expressed in Saccharomyces cerevisiae or Escherichia coli were identified by site-directed mutagenesis [145],... 

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