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Semi-random mutagenesis

A. P. Arkin and D. C. Youvan, Optimizing nucleotide mixtures to encode specific subsets of amino acids for semi-random mutagenesis, Biotechnology (NY) 1992, 10, 297-300. [Pg.335]

Figure 3.1 Overview of DNA library creation strategies. Random mutagenesis introduces mutations at positions throughout the gene sequence. Semi-rational design randomizes only the specific position(s) of interest. Gene shuffling brings existing sequence diversity from different parental DNA sequences together to form a chimeric library... Figure 3.1 Overview of DNA library creation strategies. Random mutagenesis introduces mutations at positions throughout the gene sequence. Semi-rational design randomizes only the specific position(s) of interest. Gene shuffling brings existing sequence diversity from different parental DNA sequences together to form a chimeric library...
Black ME, Kokoris MS, Sabo P. Herpes simplex virus-1 thymidine kinase mutants created by semi-random sequence mutagenesis improve prodrug-medialed tumor cell killing. Cancer Res 2001 61(7) 3022-3026. [Pg.315]

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Mutagenesis

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